Validation Data Gallery
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Tested Applications
| Positive WB detected in | COLO 320 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
19675-1-AP targets HSPG2-Specific in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | heparan sulfate proteoglycan 2 |
| Calculated molecular weight | 469 kDa |
| Observed molecular weight | 469 kDa |
| GenBank accession number | NM_005529 |
| Gene Symbol | HSPG2 |
| Gene ID (NCBI) | 3339 |
| RRID | AB_10638605 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P98160 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HSPG2, also named as PLC and HSPG, is an integral component of basement membranes. It is responsible for the fixed negative electrostatic charge and is involved in the charge-selective ultrafiltration properties. It serves as an attachment substrate for cells. Defects in HSPG2 are the cause of Schwartz-Jampel syndrome (SJS1). Defects in HSPG2 are the cause of dyssegmental dysplasia Silverman-Handmaker type (DDSH). The antibody is specific to HSPG2.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HSPG2-Specific antibody 19675-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Biol Sci Exosomal Transfer of Vasorin Expressed in Hepatocellular Carcinoma Cells Promotes Migration of Human Umbilical Vein Endothelial Cells. | ||
Nat Commun An adeno-associated virus variant enabling efficient ocular-directed gene delivery across species |