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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TIP30 Polyclonal antibody (10251-1-AP)

    WB analysis of HeLa using 10251-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10251-1-AP (TIP30 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SW 1990 cells using TIP30 Polyclonal antibody (10251-1-AP)

    WB analysis of SW 1990 using 10251-1-AP

    SW 1990 cells were subjected to SDS PAGE followed by western blot with 10251-1-AP (TIP30 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using TIP30 Polyclonal antibody (10251-1-AP)

    IHC staining of human liver using 10251-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10251-1-AP (TIP30 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using TIP30 Polyclonal antibody (10251-1-AP)

    IHC staining of human liver using 10251-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10251-1-AP (TIP30 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TIP30 Polyclonal antibody (10251-1-AP)

    IHC staining of human liver cancer using 10251-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10251-1-AP (TIP30 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TIP30 Polyclonal antibody (10251-1-AP)

    IHC staining of human liver cancer using 10251-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10251-1-AP (TIP30 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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TIP30 Polyclonal antibody

TIP30 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10251-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

CC3, HTATIP2, Oxidoreductase HTATIP2, SDR44U1, TIP30

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, SW 1990 cells
Positive IHC detected inhuman liver tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10251-1-AP targets TIP30 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TIP30 fusion protein Ag0353 相同性解析による交差性が予測される生物種
Full Name HIV-1 Tat interactive protein 2, 30kDa
Calculated molecular weight 27 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC002439
Gene Symbol TIP30
Gene ID (NCBI) 10553
RRIDAB_2279817
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BUP3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TIP30 antibody 10251-1-APDownload protocol
IHC protocol for TIP30 antibody 10251-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Cell Dev Biol

Role of GRK2 in Trophoblast Necroptosis and Spiral Artery Remodeling: Implications for Preeclampsia Pathogenesis.

Authors - Zi Lv
View Article