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KD/KO Validated

HTATSF1 Polyclonal antibody

Name: HTATSF1 Polyclonal antibody
Brand: Proteintech
SKU: 20805-1-AP

HTATSF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 20805-1-AP

Synonyms

Tat-SF1, TAT SF1, HIV Tat-specific factor 1, 17S U2 SnRNP complex component HTATSF1

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of HeLa using 20805-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 20805-1-AP (HTATSF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 20805-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 20805-1-AP (HTATSF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 20805-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 20805-1-AP (HTATSF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 20805-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 20805-1-AP (HTATSF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 20805-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 20805-1-AP (HTATSF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 20805-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 20805-1-AP (HTATSF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 20805-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 20805-1-AP (HTATSF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 20805-1-AP
IP result of anti-HTATSF1 (IP:20805-1-AP, 4ug; Detection:20805-1-AP 1:2000) with HepG2 cells lysate 2400ug.
IHC staining of human breast cancer using 20805-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 20805-1-AP (HTATSF1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human breast cancer using 20805-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 20805-1-AP (HTATSF1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human brain using 20805-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 20805-1-AP (HTATSF1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human brain using 20805-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 20805-1-AP (HTATSF1 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HeLa using 20805-1-AP
Immunofluorescent analysis of Hela cells, using HTATSF1 antibody 20805-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IF Staining of HeLa using 20805-1-AP
Immunofluorescent analysis of Hela cells, using HTATSF1 antibody 20805-1-AP at 1:50 dilution and FITC- labeled FITC-Donkey anti-Rabbit IgG (green).

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Cat No. 20805-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, mouse brain tissue, HepG2 cells, rat brain tissue, Jurkat cells
Positive IP detected in	HepG2 cells
Positive IHC detected in	human breast cancer tissue, human brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Hela cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 3 publications below
IHC	See 1 publications below
IP	See 1 publications below

Product Information

20805-1-AP targets HTATSF1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HTATSF1 fusion protein Ag14744 相同性解析による交差性が予測される生物種
Full Name	HIV-1 Tat specific factor 1
Calculated molecular weight	755 aa, 86 kDa
Observed molecular weight	130-140 kDa
GenBank accession number	BC009896
Gene symbol	HTATSF1
Gene ID (NCBI)	27336
RRID	AB_10695767
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for HTATSF1 antibody 20805-1-AP	Download protocol
IHC protocol for HTATSF1 antibody 20805-1-AP	Download protocol
IF protocol for HTATSF1 antibody 20805-1-AP	Download protocol
IP protocol for HTATSF1 antibody 20805-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cell A PARylation-phosphorylation cascade promotes TOPBP1 loading and RPA-RAD51 exchange in homologous recombination. Authors - Jiao Zhao KD Validated View Article
		Nucleic Acids Res Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II. Authors - Xiaodong Lu View Article
human	WB,IHC,IP	Cell Death Discov Metabolic protein phosphoglycerate kinase 1 confers lung cancer migration by directly binding HIV Tat specific factor 1. Authors - Yu-Chan Chang KD Validated View Article
human	WB	Cell Rep ArreSTick motif controls β-arrestin-binding stability and extends phosphorylation-dependent β-arrestin interactions to non-receptor proteins Authors - András Dávid Tóth View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HTATSF1 Polyclonal antibody

HTATSF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HeLa using 20805-1-AP

WB analysis of rat brain using 20805-1-AP

WB analysis of HepG2 using 20805-1-AP

WB analysis of Jurkat using 20805-1-AP

WB analysis of mouse brain using 20805-1-AP

WB analysis of HepG2 using 20805-1-AP

WB analysis of HeLa using 20805-1-AP

IP experiment of HepG2 using 20805-1-AP

IHC staining of human breast cancer using 20805-1-AP

IHC staining of human breast cancer using 20805-1-AP

IHC staining of human brain using 20805-1-AP

IHC staining of human brain using 20805-1-AP

IF Staining of HeLa using 20805-1-AP

IF Staining of HeLa using 20805-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

A PARylation-phosphorylation cascade promotes TOPBP1 loading and RPA-RAD51 exchange in homologous recombination.

Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II.

Metabolic protein phosphoglycerate kinase 1 confers lung cancer migration by directly binding HIV Tat specific factor 1.

ArreSTick motif controls β-arrestin-binding stability and extends phosphorylation-dependent β-arrestin interactions to non-receptor proteins