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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

5HT2A Receptor Polyclonal antibody

5HT2A Receptor Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26438-1-AP

Synonyms

5 HT 2, 5 HT 2A, 5 HT2A, 5HT2A Receptor, HTR2, HTR2A, Serotonin receptor 2A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using 5HT2A Receptor Polyclonal antibody (26438-1-AP)

    WB analysis using 26438-1-AP

    H9C2 cells were subjected to SDS PAGE followed by western blot with 26438-1-AP (5HT2A Receptor antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using 5HT2A Receptor Polyclonal antibody (26438-1-AP)

    IHC staining of human brain using 26438-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26438-1-AP (5HT2A Receptor Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using 5HT2A Receptor Polyclonal antibody (26438-1-AP)

    IHC staining of human brain using 26438-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26438-1-AP (5HT2A Receptor Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using 5HT2A Receptor Polyclonal antibody (26438-1-AP)

    IHC staining of human small intestine using 26438-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 26438-1-AP (5HT2A Receptor Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using 5HT2A Receptor Polyclonal antibody (26438-1-AP)

    IHC staining of human small intestine using 26438-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 26438-1-AP (5HT2A Receptor Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inH9C2 cells
Positive IHC detected inhuman brain tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26438-1-AP targets 5HT2A Receptor in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen 5HT2A Receptor fusion protein Ag24056 相同性解析による交差性が予測される生物種
Full Name 5-hydroxytryptamine (serotonin) receptor 2A
Calculated molecular weight 471 aa, 53 kDa
Observed molecular weight53 kDa
GenBank accession numberBC074848
Gene symbol 5HT2A Receptor
Gene ID (NCBI) 3356
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The 5HT2A Receptor gene (HTR2A) codes for a G-protein coupled receptor and belongs to the G-protein coupled receptor 1 family. HTR2A plays a key role in serotonin pathway regulation. HTR2A affects neural activity, perception, cognition, and mood and functions as the main target of atypical antipsychotic drugs (PMID: 17691947, 21550210). 5HT2A Receptor has 2 isoforms with the molecular mass of 44 and 53 kDa.

Protocols

Product Specific Protocols
WB protocol for 5HT2A Receptor antibody 26438-1-APDownload protocol
IHC protocol for 5HT2A Receptor antibody 26438-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols