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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

5HT2A Receptor Polyclonal antibody

5HT2A Receptor Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26438-1-AP

Synonyms

5 HT 2, 5 HT 2A, 5 HT2A, 5HT2A Receptor, HTR2, HTR2A, Serotonin receptor 2A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using 5HT2A Receptor Polyclonal antibody (26438-1-AP)

    WB analysis using 26438-1-AP

    H9C2 cells were subjected to SDS PAGE followed by western blot with 26438-1-AP (5HT2A Receptor antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using 5HT2A Receptor Polyclonal antibody (26438-1-AP)

    IHC staining of human brain using 26438-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26438-1-AP (5HT2A Receptor Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using 5HT2A Receptor Polyclonal antibody (26438-1-AP)

    IHC staining of human brain using 26438-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26438-1-AP (5HT2A Receptor Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using 5HT2A Receptor Polyclonal antibody (26438-1-AP)

    IHC staining of human small intestine using 26438-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 26438-1-AP (5HT2A Receptor Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using 5HT2A Receptor Polyclonal antibody (26438-1-AP)

    IHC staining of human small intestine using 26438-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 26438-1-AP (5HT2A Receptor Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inH9C2 cells
Positive IHC detected inhuman brain tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26438-1-AP targets 5HT2A Receptor in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen 5HT2A Receptor fusion protein Ag24056 相同性解析による交差性が予測される生物種
Full Name 5-hydroxytryptamine (serotonin) receptor 2A
Calculated molecular weight 471 aa, 53 kDa
Observed molecular weight53 kDa
GenBank accession numberBC074848
Gene symbol 5HT2A Receptor
Gene ID (NCBI) 3356
RRIDAB_3085872
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The 5HT2A Receptor gene (HTR2A) codes for a G-protein coupled receptor and belongs to the G-protein coupled receptor 1 family. HTR2A plays a key role in serotonin pathway regulation. HTR2A affects neural activity, perception, cognition, and mood and functions as the main target of atypical antipsychotic drugs (PMID: 17691947, 21550210). 5HT2A Receptor has 2 isoforms with the molecular mass of 44 and 53 kDa.

Protocols

Product Specific Protocols
WB protocol for 5HT2A Receptor antibody 26438-1-APDownload protocol
IHC protocol for 5HT2A Receptor antibody 26438-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols