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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HTR3A Polyclonal antibody (29685-1-AP)

    WB analysis using 29685-1-AP (same clone as 29685-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29685-1-AP (HTR3A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29685-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using HTR3A Polyclonal antibody (29685-1-AP)

    IHC staining of human small intestine using 29685-1-AP (same clone as 29685-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 29685-1-AP (HTR3A antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 29685-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using HTR3A Polyclonal antibody (29685-1-AP)

    IHC staining of human small intestine using 29685-1-AP (same clone as 29685-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 29685-1-AP (HTR3A antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 29685-1-PBS in a different storage buffer formulation.

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HTR3A Polyclonal antibody, PBS Only

HTR3A Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29685-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29685-1-PBS targets HTR3A in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HTR3A fusion protein Ag31317 相同性解析による交差性が予測される生物種
Full Name 5-hydroxytryptamine (serotonin) receptor 3A
Calculated molecular weight 55 kDa
Observed molecular weight55 kDa
GenBank accession numberBC002354
Gene Symbol HTR3A
Gene ID (NCBI) 3359
RRIDAB_2918338
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP46098
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HTR3A (5-hydroxytryptamine receptor 3A, 5-HT3A) belongs to the ligand-gated ion channel receptor superfamily. It is the subunit A of the type 3 receptor for 5-hydroxytryptamine (5-HT, serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. So far, five distinct 5-HT3 receptor subunits (A-E) have been identified. HTR3A can form functional homomers or heteromers with HTR3B or HTR3C or HTR3D or HTR3E. 5-HT3 receptors are capable of mediating fast excitatory neurotransmission in the CNS and peripheral nervous system (PNS). (PMID: 23038271; 17073663)