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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using HTR7 Polyclonal antibody (13830-1-AP)

    WB analysis of mouse brain using 13830-1-AP (same clone as 13830-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13830-1-AP (HTR7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13830-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse spleen tissue using HTR7 Polyclonal antibody (13830-1-AP)

    WB analysis of mouse spleen using 13830-1-AP (same clone as 13830-1-PBS)

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13830-1-AP (HTR7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13830-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using HTR7 Polyclonal antibody (13830-1-AP)

    IHC staining of human brain using 13830-1-AP (same clone as 13830-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13830-1-AP (HTR7 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 13830-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using HTR7 Polyclonal antibody (13830-1-AP)

    IHC staining of human brain using 13830-1-AP (same clone as 13830-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13830-1-AP (HTR7 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 13830-1-PBS in a different storage buffer formulation.

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HTR7 Polyclonal antibody, PBS Only

HTR7 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 13830-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13830-1-PBS targets HTR7 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HTR7 fusion protein Ag4742 相同性解析による交差性が予測される生物種
Full Name 5-hydroxytryptamine (serotonin) receptor 7 (adenylate cyclase-coupled)
Calculated molecular weight 445 aa, 49 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC047526
Gene Symbol HTR7
Gene ID (NCBI) 3363
RRIDAB_2122706
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP34969
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Serotonin (5-hydroxytryptamine; 5-HT) acts as a neurotransmitter and neuromodulator in the central nervous system(CNS) (PMID:23100172). The behavioral effects of 5-HT are mediated by a large family of specific 5-HT receptors. HTR7 (5-hydroxytryptamine receptor 7) is a G protein-coupled receptor (GPCR), and many transcription factors can bind to its promoter, such as AP2, EGR-1, and MAZ (PMID:34164474). It is located in different brain regions, such as the hypothalamus, thalamus, cerebral cortex, and hippocampus (PMID:29637287). HTR7 is associated with circadian rhythm, anxiety, depression, schizophrenia, nociception, and memory (PMID:9154233).