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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using HTR7 Polyclonal antibody (29238-1-AP)

    IHC staining of mouse cerebellum using 29238-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29238-1-AP (HTR7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using HTR7 Polyclonal antibody (29238-1-AP)

    IHC staining of mouse brain using 29238-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29238-1-AP (HTR7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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HTR7 Polyclonal antibody

HTR7 Polyclonal Antibody for IHC, ELISA
Cat No. 29238-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Synonyms

5-HT-7, 5-HT7, 5 hydroxytryptamine receptor 7, 5 HT7, 5 HT X

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inmouse cerebellum tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29238-1-AP targets HTR7 in IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HTR7 fusion protein Ag30856 相同性解析による交差性が予測される生物種
Full Name 5-hydroxytryptamine (serotonin) receptor 7 (adenylate cyclase-coupled)
Calculated molecular weight 445 aa, 49 kDa
GenBank accession numberBC047526
Gene Symbol HTR7
Gene ID (NCBI) 3363
RRIDAB_3669679
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP34969
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The 5-hydroxytryptamine receptor 7 (HTR7), also known as 5-HT7, is a G protein-coupled receptor that is activated by the neurotransmitter serotonin (5-HT). It is involved in the G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger and chemical synaptic transmission. It is expressed in several structures, including adipose tissue, the alimentary system, the brain, the immune system, and the reproductive system.

Protocols

Product Specific Protocols
IHC protocol for HTR7 antibody 29238-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols