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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HTRA1 Monoclonal antibody (68053-1-Ig)

    WB analysis using 68053-1-Ig (same clone as 68053-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68053-1-Ig (HTRA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68053-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using HTRA1 Monoclonal antibody (68053-1-Ig)

    WB analysis using 68053-1-Ig (same clone as 68053-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68053-1-Ig (HTRA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68053-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using HTRA1 Monoclonal antibody (68053-1-Ig)

    FC experiment of HeLa using 68053-1-Ig (same clone as 68053-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug HTRA1 Monoclonal antibody (68053-1-Ig, Clone:1B11G12) and CoraLite488-conjugated Donkey Anti-Mouse IgG(H+L)( SA00013-5)(red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68053-1-PBS in a different storage buffer formulation.

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HTRA1 Monoclonal antibody, PBS Only

HTRA1 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68053-1-PBS
Clone No.1B11G12

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, Indirect ELISA

Synonyms

PRSS11, HtrA, EC:3.4.21.-, 1B11G12

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68053-1-PBS targets HTRA1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HTRA1 fusion protein Ag31204 相同性解析による交差性が予測される生物種
Full Name HtrA serine peptidase 1
Calculated molecular weight 51 kDa
Observed molecular weight 51 kDa
GenBank accession numberNM_002775
Gene Symbol HTRA1
Gene ID (NCBI) 5654
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92743
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HTRA1, also named as HTRA, PRSS11 and L56, belongs to the peptidase S1B family. It is a protease that regulates the availability of nsulin-like growth factors (IGFs) by cleaving IGF-binding proteins. HTRA1 represses signaling by TGF-beta family members. Variations in the promoter region of HTRA1 are the cause of susceptibility to age-related macular degeneration type 7 (ARMD7). Defects in HTRA1 are the cause of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL). The 50 kDa HTRA1 was found at varying levels in the majority samples tested, whereas the 42 kDa form of HTRA1 was identified in degenerated intervertebral disc protein samples(PMID:22556410). The short truncated monomer 37 kDa and dimer 74 kDa are proved (PMID:25329061). The antibody is specific to HTRA1.