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  • Featured Product
  • KD/KO Validated

HTRA2 Polyclonal antibody, PBS Only

HTRA2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15775-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using HTRA2 Polyclonal antibody (15775-1-AP)

    WB analysis of HeLa using 15775-1-AP (same clone as 15775-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 15775-1-AP (HTRA2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using HTRA2 Polyclonal antibody (15775-1-AP)

    WB analysis of human heart using 15775-1-AP (same clone as 15775-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 15775-1-AP (HTRA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using HTRA2 Polyclonal antibody (15775-1-AP)

    WB analysis of mouse skeletal muscle using 15775-1-AP (same clone as 15775-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15775-1-AP (HTRA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using HTRA2 Polyclonal antibody (15775-1-AP)

    WB analysis of Raji using 15775-1-AP (same clone as 15775-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 15775-1-AP (HTRA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Raji cells using HTRA2 Polyclonal antibody (15775-1-AP)

    IP experiment of Raji using 15775-1-AP (same clone as 15775-1-PBS)

    IP result of anti-HTRA2 (IP:15775-1-AP, 4ug; Detection:15775-1-AP 1:500) with Raji cells lysate 2000ug. This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using HTRA2 Polyclonal antibody (15775-1-AP)

    IHC staining of human kidney using 15775-1-AP (same clone as 15775-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 15775-1-AP (HTRA2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using HTRA2 Polyclonal antibody (15775-1-AP)

    IHC staining of human kidney using 15775-1-AP (same clone as 15775-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 15775-1-AP (HTRA2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using HTRA2 Polyclonal antibody (15775-1-AP)

    IHC staining of human colon using 15775-1-AP (same clone as 15775-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15775-1-AP (HTRA2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using HTRA2 Polyclonal antibody (15775-1-AP)

    IHC staining of human colon using 15775-1-AP (same clone as 15775-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15775-1-AP (HTRA2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using HTRA2 Polyclonal antibody (15775-1-AP)

    IHC staining of human colon using 15775-1-AP (same clone as 15775-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15775-1-AP (HTRA2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using HTRA2 Polyclonal antibody (15775-1-AP)

    IHC staining of human colon using 15775-1-AP (same clone as 15775-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15775-1-AP (HTRA2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid tissue using HTRA2 Polyclonal antibody (15775-1-AP)

    IHC staining of human thyroid using 15775-1-AP (same clone as 15775-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 15775-1-AP (HTRA2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid tissue using HTRA2 Polyclonal antibody (15775-1-AP)

    IHC staining of human thyroid using 15775-1-AP (same clone as 15775-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 15775-1-AP (HTRA2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15775-1-PBS targets HTRA2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HTRA2 fusion protein Ag8455 相同性解析による交差性が予測される生物種
Full Name HtrA serine peptidase 2
Calculated molecular weight 49 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC000096
Gene symbol HTRA2
Gene ID (NCBI) 27429
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HTRA2(High temperature requirement protein A2) is also named as OMI, PRSS25 and belongs to the peptidase S1B family. HTRA2 normally exists in the mitochondria, can cause MMP3 activation in the cytosol under a cell stress condition, which can ultimately lead to demise of dopaminergic neuronal cells(PMID: 22265821). It inhibits mitochondrial superoxide generation, stabilizes mitochondrial membrane potential, and prevents apoptosis at baseline and in response to extracellular inducers of mitochondrial stress(PMID: 18772386). This protein has 4 isoforms produced by alternative splicing.