Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of HeLa using 15775-1-AP (same clone as 15775-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 15775-1-AP (HTRA2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.
WB analysis of human heart using 15775-1-AP (same clone as 15775-1-PBS)
human heart tissue were subjected to SDS PAGE followed by western blot with 15775-1-AP (HTRA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.
WB analysis of mouse skeletal muscle using 15775-1-AP (same clone as 15775-1-PBS)
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15775-1-AP (HTRA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.
WB analysis of Raji using 15775-1-AP (same clone as 15775-1-PBS)
Raji cells were subjected to SDS PAGE followed by western blot with 15775-1-AP (HTRA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.
IP experiment of Raji using 15775-1-AP (same clone as 15775-1-PBS)
IP result of anti-HTRA2 (IP:15775-1-AP, 4ug; Detection:15775-1-AP 1:500) with Raji cells lysate 2000ug. This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 15775-1-AP (same clone as 15775-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 15775-1-AP (HTRA2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 15775-1-AP (same clone as 15775-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 15775-1-AP (HTRA2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 15775-1-AP (same clone as 15775-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15775-1-AP (HTRA2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 15775-1-AP (same clone as 15775-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15775-1-AP (HTRA2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.
IHC staining of human thyroid using 15775-1-AP (same clone as 15775-1-PBS)
Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 15775-1-AP (HTRA2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.
IHC staining of human thyroid using 15775-1-AP (same clone as 15775-1-PBS)
Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 15775-1-AP (HTRA2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 15775-1-AP (same clone as 15775-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15775-1-AP (HTRA2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 15775-1-AP (same clone as 15775-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15775-1-AP (HTRA2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

HTRA2 Polyclonal antibody, PBS Only

HTRA2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 15775-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15775-1-PBS targets HTRA2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity	human, mouse, rat, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HTRA2 fusion protein Ag8455 相同性解析による交差性が予測される生物種
Full Name	HtrA serine peptidase 2
Calculated molecular weight	49 kDa
Observed molecular weight	36 kDa
GenBank accession number	BC000096
Gene Symbol	HTRA2
Gene ID (NCBI)	27429
RRID	AB_2122835
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O43464
Storage Buffer	PBS only
Storage Conditions	Store at -80°C.

Background Information

HTRA2(High temperature requirement protein A2) is also named as OMI, PRSS25 and belongs to the peptidase S1B family. HTRA2 normally exists in the mitochondria, can cause MMP3 activation in the cytosol under a cell stress condition, which can ultimately lead to demise of dopaminergic neuronal cells(PMID: 22265821). It inhibits mitochondrial superoxide generation, stabilizes mitochondrial membrane potential, and prevents apoptosis at baseline and in response to extracellular inducers of mitochondrial stress(PMID: 18772386). This protein has 4 isoforms produced by alternative splicing.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 15775-1-AP (same clone as 15775-1-PBS)

WB analysis of human heart using 15775-1-AP (same clone as 15775-1-PBS)

WB analysis of mouse skeletal muscle using 15775-1-AP (same clone as 15775-1-PBS)

WB analysis of Raji using 15775-1-AP (same clone as 15775-1-PBS)

IP experiment of Raji using 15775-1-AP (same clone as 15775-1-PBS)

IHC staining of human kidney using 15775-1-AP (same clone as 15775-1-PBS)

IHC staining of human kidney using 15775-1-AP (same clone as 15775-1-PBS)

IHC staining of human colon using 15775-1-AP (same clone as 15775-1-PBS)

IHC staining of human colon using 15775-1-AP (same clone as 15775-1-PBS)

IHC staining of human thyroid using 15775-1-AP (same clone as 15775-1-PBS)

IHC staining of human thyroid using 15775-1-AP (same clone as 15775-1-PBS)

IHC staining of human colon using 15775-1-AP (same clone as 15775-1-PBS)

IHC staining of human colon using 15775-1-AP (same clone as 15775-1-PBS)

HTRA2 Polyclonal antibody, PBS Only

HTRA2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information