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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HTRA2 Recombinant antibody, PBS Only

HTRA2 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240496D1

Cat no : 83492-7-PBS

Synonyms

PARK13, Omi stress-regulated endoprotease, OMI, HtrA serine peptidase 2, EC:3.4.21.108

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HTRA2 Recombinant antibody (83492-7-RR)

    WB analysis using 83492-7-RR (same clone as 83492-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83492-7-RR (HTRA2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83492-7-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HTRA2 Recombinant antibody (83492-7-RR)

    IF Staining of HepG2 using 83492-7-RR (same clone as 83492-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HTRA2 antibody (83492-7-RR, Clone: 240496D1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83492-7-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using HTRA2 Recombinant antibody (83492-7-RR)

    FC experiment of HeLa using 83492-7-RR (same clone as 83492-7-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HTRA2 Recombinant antibody (83492-7-RR, Clone:240496D1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83492-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83492-7-RR

    Affinity and Kinetic Characterization of 83492-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83492-7-RR against Human HTRA2 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83492-7-PBS is HTRA2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HTRA2 fusion protein Ag8455 相同性解析による交差性が予測される生物種
Full Name HtrA serine peptidase 2
Calculated molecular weight 49 kDa
GenBank accession numberBC000096
Gene symbol HTRA2
Gene ID (NCBI) 27429
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.