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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Huntingtin Polyclonal antibody

Huntingtin Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19957-1-AP

Synonyms

HTT, IT15, Huntington disease protein, Huntingtin, myristoylated N-terminal fragment, HD protein

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse brain tissue using Huntingtin Polyclonal antibody (19957-1-AP)

    IHC staining of mouse brain using 19957-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19957-1-AP (Huntingtin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Huntingtin Polyclonal antibody (19957-1-AP)

    IHC staining of mouse brain using 19957-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19957-1-AP (Huntingtin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Huntingtin Polyclonal antibody (19957-1-AP)

    IHC staining of mouse brain using 19957-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19957-1-AP (Huntingtin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Huntingtin Polyclonal antibody (19957-1-AP)

    IHC staining of mouse brain using 19957-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19957-1-AP (Huntingtin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using Huntingtin Polyclonal antibody (19957-1-AP)

    IF Staining of HEK-293 using 19957-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using Huntingtin antibody (19957-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using Huntingtin Polyclonal antibody (19957-1-AP)

    IF Staining of SH-SY5Y using 19957-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Huntingtin antibody (19957-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19957-1-AP targets Huntingtin in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name huntingtin
Calculated molecular weight 348 kDa
GenBank accession numberNM_002111
Gene symbol Huntingtin
Gene ID (NCBI) 3064
RRIDAB_2878629
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HTT, also named as HD and IT15, belongs to the huntingtin family. HTT may play a role in microtubule-mediated transport or vesicle function. Defects in HTT are the cause of Huntington disease (HD) which is an autosomal dominant neurodegenerative disorder characterized by involuntary movements (chorea), general motor impairment, psychiatric disorders and dementia. The antibody recognizes the C-term of HTT.

Protocols

Product Specific Protocols
IHC protocol for Huntingtin antibody 19957-1-APDownload protocol
IF protocol for Huntingtin antibody 19957-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols