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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HUS1 Monoclonal antibody, PBS Only

HUS1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E8E9

Cat no : 67168-1-PBS

Synonyms

Checkpoint protein HUS1, hHUS1, HUS1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using HUS1 Monoclonal antibody (67168-1-Ig)

    WB analysis using 67168-1-Ig (same clone as 67168-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67168-1-Ig (HUS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67168-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using HUS1 Monoclonal antibody (67168-1-Ig)

    IHC staining of human prostate cancer using 67168-1-Ig (same clone as 67168-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67168-1-Ig (HUS1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67168-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using HUS1 Monoclonal antibody (67168-1-Ig)

    IHC staining of human prostate cancer using 67168-1-Ig (same clone as 67168-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67168-1-Ig (HUS1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67168-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67168-1-PBS targets HUS1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HUS1 fusion protein Ag28527 相同性解析による交差性が予測される生物種
Full Name HUS1 checkpoint homolog (S. pombe)
Calculated molecular weight 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC007013
Gene symbol HUS1
Gene ID (NCBI) 3364
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Hus1 belongs to the Rad family, form a heterotrimeric clamp with Rad1and Rad9 that acts as a putative sensor for DNA damage. This complex plays an apical role in the DNA damage response, loss of a functional complex affects many downstream checkpoint pathways [PMID: 11340080]. It is also a key complex involved in the activation of the ATR pathway. 9-1-1 forms a ring-shaped heterotrimer, resembling the homotrimeric sliding clamp, proliferating cell nuclear antigen (PCNA) [PMID: 19535328].