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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HVCN1 Polyclonal antibody

HVCN1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14162-1-AP

Synonyms

VSOP, Voltage-gated hydrogen channel 1, Voltage sensor domain-only protein, UNQ578/PRO1140, Hydrogen voltage-gated channel 1

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using HVCN1 Polyclonal antibody (14162-1-AP)

    WB analysis of Raji using 14162-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 14162-1-AP (HVCN1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using HVCN1 Polyclonal antibody (14162-1-AP)

    WB analysis of PC-3 using 14162-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 14162-1-AP (HVCN1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using HVCN1 Polyclonal antibody (14162-1-AP)

    WB analysis of Raji using 14162-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 14162-1-AP (HVCN1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of PC-3 cells using HVCN1 Polyclonal antibody (14162-1-AP)

    IP experiment of PC-3 using 14162-1-AP

    IP result of anti-HVCN1 (IP:14162-1-AP, 4ug; Detection:14162-1-AP 1:300) with PC-3 cells lysate 4000 ug.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HVCN1 Polyclonal antibody (14162-1-AP)

    IHC staining of human tonsillitis using 14162-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14162-1-AP (HVCN1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HVCN1 Polyclonal antibody (14162-1-AP)

    IHC staining of human tonsillitis using 14162-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14162-1-AP (HVCN1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HVCN1 Polyclonal antibody (14162-1-AP)

    IHC staining of human tonsillitis using 14162-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14162-1-AP (HVCN1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using HVCN1 Polyclonal antibody (14162-1-AP)

    IF Staining of PC-3 using 14162-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 14162-1-AP (HVCN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inRaji cells, PC-3 cells
Positive IP detected inPC-3 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

14162-1-AP targets HVCN1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HVCN1 fusion protein Ag5350 相同性解析による交差性が予測される生物種
Full Name hydrogen voltage-gated channel 1
Calculated molecular weight 273 aa, 32 kDa
Observed molecular weight 28-32 kDa, ~60 kDa
GenBank accession numberBC032672
Gene symbol HVCN1
Gene ID (NCBI) 84329
RRIDAB_2878023
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HVCN1, also named as VSOP and HV1, Belongs to the hydrogen channel family. HVCN1 mediates the voltage-dependent proton permeability of excitable membranes. It forms a proton-selective channel through which protons may pass in accordance with their electrochemical gradient. Proton efflux, HVCN1 is accompanied by membrane depolarization, facilitates acute production of reactive oxygen species in phagocytosis. HVCN1, the voltage-sensitive proton channel, is present in human sperm and is an important regulator of the functional maturation of sperm. HVCN1 has four isoforms with MW 28-32 kDa or 40 kDa (modification). It has a dimer form with MW ~60 kDa.

Protocols

Product Specific Protocols
WB protocol for HVCN1 antibody 14162-1-APDownload protocol
IHC protocol for HVCN1 antibody 14162-1-APDownload protocol
IF protocol for HVCN1 antibody 14162-1-APDownload protocol
IP protocol for HVCN1 antibody 14162-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Immunol

Diagnostic and Predictive Value of Immune-Related Genes in Crohn's Disease.

Authors - Bing Yu
View Article