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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HYAL1 Polyclonal antibody

HYAL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25179-1-AP

Synonyms

HYAL 1, HYAL1, Hyaluronidase 1, hyaluronoglucosaminidase 1, LuCa 1, LUCA1, Lung carcinoma protein 1, NAT6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HYAL1 Polyclonal antibody (25179-1-AP)

    WB analysis using 25179-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25179-1-AP (HYAL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using HYAL1 Polyclonal antibody (25179-1-AP)

    IHC staining of mouse liver using 25179-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25179-1-AP (HYAL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using HYAL1 Polyclonal antibody (25179-1-AP)

    IHC staining of mouse liver using 25179-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25179-1-AP (HYAL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHCT 116 cells, HeLa cells, HepG2 cells, MCF-7 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25179-1-AP targets HYAL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HYAL1 fusion protein Ag17943 相同性解析による交差性が予測される生物種
Full Name hyaluronoglucosaminidase 1
Calculated molecular weight 435 aa, 48 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC035695
Gene symbol HYAL1
Gene ID (NCBI) 3373
RRIDAB_2879945
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Hyaluronic acid (HA) is a glycosaminoglycan that is believed to have numerous important biologic functions, including modulation of cell proliferation, migration, and differentiation, as well as the regulation of extracellular water and protein homeostasis. It is also an integral structural component of cartilage and other tissues and acts as a lubricant in joints. Hyaluronidases are a family of enzymes that catalyse the degradation of HA. In humans, there are five functional hyaluronidases: HYAL1, HYAL2, HYAL3, HYAL4 and HYAL5 (also known as SPAM1 or PH-20); plus a pseudogene, HYAL6 (also known as HYALP1). HYAL-1 is present in many tissues and is predominantly found in the plasma and urine (PMID: 16600643). In addition to its function in normal cellular hyaluronan turnover, human HYAL-1 is implicated in cancer proliferation, angiogenesis, and inflammatory diseases (PMID: 17503783).

Protocols

Product Specific Protocols
WB protocol for HYAL1 antibody 25179-1-APDownload protocol
IHC protocol for HYAL1 antibody 25179-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols