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ChromoTek mouse anti Halo Monoclonal antibody (28A8)

Mouse monoclonal antibody [28A8] to Halo-tag

Host/IsoType

Mouse/IgG1

Specificity

Halo-tag

Applications

WB

Conjugate

Unconjugated

Clone No.

28A8

Cat no : 28a8

Synonyms

Halo tag, dehalogenase, Halo

Validation Data Gallery

  • Western blot analysis of cell extract from HEK293T cells transiently expressing Actin-Chromobody-EGFP-Halo-tag and from untransfected cells.

    Western blot analysis of cell extract from HEK293T cells transiently expressing Actin-Chromobody-EGFP-Halo-tag and from untransfected cells.

  • Sensitivity tested with purified recombinant Halo-tag protein. Primary antibody: anti-Halo 1:500. Secondary antibody: anti-mouse Alexa Fluor? 647 1:1,000.

    Sensitivity tested with purified recombinant Halo-tag protein. Primary antibody: anti-Halo 1:500. Secondary antibody: anti-mouse Alexa Fluor? 647 1:1,000.

Product Information

Mouse monoclonal antibody [28A8] to Halo-tag

DescriptionHalo mouse monoclonal [28A8] for WB
ApplicationsWB
Specificity/TargetHalo-tag, derived from the bacterial haloalkane dehalogenase enzyme from Rhodococcus rhodochrous
Recommended DilutionWestern Blot: 1:500
Host/IsoTypeMouse/IgG1
ClassMonoclonal
TypePrimary Antibody
UniProtKBP0A3G2
Clone No.28A8
ConjugateUnconjugated
FormLiquid
Purification MethodProtein A purified
RRIDAB_2827565
Storage BufferPBS, 50% glycerol, 0.09% sodium azide
Storage ConditionShipped on blue ice. Store at -20°C. Stable for one year.
Size20 μL; 100 μL

Publications

ApplicationTitle

bioRxiv

An approach to produce thousands of single-chain antibody variants on a SPR biosensor chip for measuring target binding kinetics and for deep characterization of antibody paratopes

Authors - Rebecca L Cook
View Article
WB

G3 (Bethesda)

A set of Saccharomyces cerevisiae integration vectors for fluorescent dye labeling of proteins

Authors - Inwha Baek
View Article

Nature

PLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infection

Authors - Dijin Xu
View Article
WB

Curr Biol

A bHLH heterodimer regulates germ cell differentiation in land plant gametophytes

Authors - Misaki Saito
View Article