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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Haptoglobin Monoclonal antibody, PBS Only

Haptoglobin Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B4B9

Cat no : 66229-1-PBS

Synonyms

BP, HAPT, haptoglobin, HP, HP2 ALPHA 2, HPA1S, Zonulin

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Haptoglobin Monoclonal antibody (66229-1-Ig)

    WB analysis of human plasma using 66229-1-Ig (same clone as 66229-1-PBS)

    0.5 μl human plasma was subjected to SDS PAGE followed by western blot with 66229-1-Ig (HP Antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66229-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Haptoglobin Monoclonal antibody (66229-1-Ig)

    IHC staining of human liver cancer using 66229-1-Ig (same clone as 66229-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66229-1-Ig (Haptoglobin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66229-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Haptoglobin Monoclonal antibody (66229-1-Ig)

    IHC staining of human liver cancer using 66229-1-Ig (same clone as 66229-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66229-1-Ig (Haptoglobin antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66229-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66229-1-PBS targets Haptoglobin in WB, IHC, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Haptoglobin fusion protein Ag9927 相同性解析による交差性が予測される生物種
Full Name haptoglobin
Calculated molecular weight281aa,31 kDa; 228aa,25 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC058031
Gene symbol HP
Gene ID (NCBI) 3240
RRIDAB_2881620
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HP(Haptoglobin) is also named as zonulin and belongs to the peptidase S1 family. HP, a plasma glycoprotein that binds free hemoglobin, has a tetrameric structure of 2 alpha(16 kDa and 9 kDa) and 2 beta(40 kDa) polypeptides that are covalently associated by disulfide bonds. In most species, apart from ruminants, Hp has a molecular mass of 100 kDa, consisting of two subunits of 40 kDa and two subunits of 9 kDa, although in a few species, such as man, genetic variant of Hp forms polymers of higher mass(PMID:2361363). Recent studies of haptoglobin show that certain oligosaccharide structures predominate in different diseases. For example, a highly-fucosylated structure is found in breast cancer and ovarian cancer, highly-sialylated structures in Crohn’s disease and highly branched structures in alcoholic liver disease and fucosylated haptoglobin is a good serum marker for pancreatic cancer.(PMID:16385567).