Mouse KIM-1/HAVCR1 Recombinant antibody, PBS Only (Capture)

Sandwich ELISA standard curve of MP00116-3, Mouse KIM-1/HAVCR1Recombinant Matched Antibody Pair - PBS only. 83064-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0571. 83064-5-PBS. was HRP conjugated as the detection antibody. Range: 0.625-10 ng/mL.

Urine of five mice was measured. The Havcr1 concentration of detected samples was determined to be 12.73 ng/mL with a range of 1.61-25.85 ng/mL.

Kidneys from mice were removed, rinsed in 1X PBS, and kept on ice in 1X PBS. Kidneys were then homogenized using a tissue homogenizer and seeded into media containing RPMI-1640 supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 mg/mL streptomycin sulfate. Cells were cultured for the times indicated. The mean Havcr1 concentration was determined to be 602.5 pg/mL in Balb/c mouse kidney, 724.5 pg/mL in C57 mouse kidney.

Serum of sixteen mice was measured. The Havcr1 concentration of detected samples was determined to be 143.31 pg/mL with a range of 97.69-240.45 pg/mL

Cytometric bead array standard curve of MP00116-3, Mouse KIM-1/HAVCR1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83064-4-PBS. Detection antibody: 83064-5-PBS. Standard: Eg0571. Range: 0.625-10 ng/mL.