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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Hemopexin Monoclonal antibody (66479-1-Ig)

    WB analysis of human plasma using 66479-1-Ig (same clone as 66479-1-PBS)

    0.7 μL human plasma was subjected to SDS PAGE followed by western blot with 66479-1-Ig (Hemopexin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig plasma using Hemopexin Monoclonal antibody (66479-1-Ig)

    WB analysis of pig plasma using 66479-1-Ig (same clone as 66479-1-PBS)

    0.7 μL pig plasma was subjected to SDS PAGE followed by western blot with 66479-1-Ig (Hemopexin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat serum using Hemopexin Monoclonal antibody (66479-1-Ig)

    WB analysis of rat serum using 66479-1-Ig (same clone as 66479-1-PBS)

    0.7 μL rat serum was subjected to SDS PAGE followed by western blot with 66479-1-Ig (Hemopexin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using Hemopexin Monoclonal antibody (66479-1-Ig)

    WB analysis of human placenta using 66479-1-Ig (same clone as 66479-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66479-1-Ig (Hemopexin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Hemopexin Monoclonal antibody (66479-1-Ig)

    IHC staining of human liver using 66479-1-Ig (same clone as 66479-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66479-1-Ig (Hemopexin antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Hemopexin Monoclonal antibody (66479-1-Ig)

    IHC staining of human liver using 66479-1-Ig (same clone as 66479-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66479-1-Ig (Hemopexin antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Hemopexin Monoclonal antibody (66479-1-Ig)

    IHC staining of human placenta using 66479-1-Ig (same clone as 66479-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66479-1-Ig (Hemopexin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using Hemopexin Monoclonal antibody (66479-1-Ig)

    IF Staining of human liver cancer using 66479-1-Ig (same clone as 66479-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Hemopexin antibody (66479-1-Ig, Clone: 3A9D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using Hemopexin Monoclonal antibody (66479-1-Ig)

    FC experiment of HepG2 using 66479-1-Ig (same clone as 66479-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human Hemopexin (66479-1-Ig, Clone:3A9D6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

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Hemopexin Monoclonal antibody, PBS Only

Hemopexin Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA
Cat No. 66479-1-PBS
Clone No.3A9D6

Host / Isotype

Mouse / IgG1

Reactivity

pig, rat, human

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Synonyms

HPX, Beta-1B-glycoprotein, Beta 1B glycoprotein, 3A9D6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66479-1-PBS targets Hemopexin in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with pig, rat, human samples.

Tested Reactivity pig, rat, human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Hemopexin fusion protein Ag8533 相同性解析による交差性が予測される生物種
Full Name hemopexin
Calculated molecular weight 254 aa, 29 kDa
Observed molecular weight 65-75 kDa
GenBank accession numberBC005395
Gene Symbol Hemopexin
Gene ID (NCBI) 3263
RRIDAB_2881845
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP02790
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Hemopexin (HPX) is the plasma protein responsible for scavenging heme, thus preventing heme-mediated oxidative stress and heme-bound iron loss. In addition, hemopexin blocks heme activation of immune receptors and vascular inflammatory processes. It is mainly expressed in liver, the synthesis of which is induced after inflammation. Alterations of plasma hemopexin level have been linked to disorders like atherosclerosis and inflammatory diseases.