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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mouse HER2/ErbB2 Recombinant antibody, PBS Only

HER2/ErbB2 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241366F4

Cat no : 84128-5-PBS

Synonyms

Her2 / ErbB2, Her2, Erbb2, EC:2.7.10.1, CD340

Validation Data Gallery

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  • Western Blot (WB) analysis of 4T1 cells using HER2/ErbB2 Recombinant antibody (84128-5-RR)

    WB analysis of 4T1 using 84128-5-RR (same clone as 84128-5-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 84128-5-RR (Her2 / ErbB2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84128-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of 4T1 cells using HER2/ErbB2 Recombinant antibody (84128-5-RR)

    IF Staining of 4T1 using 84128-5-RR (same clone as 84128-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed 4T1 cells using Her2 / ErbB2 antibody (84128-5-RR, Clone: 241366F4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84128-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84128-5-RR

    Affinity and Kinetic Characterization of 84128-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84128-5-RR against Mouse Her2 / ErbB2 were performed. The affinity constant is 0.356 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 84128-5-PBS is HER2/ErbB2 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
Calculated molecular weight139 kDa
Observed molecular weight185 kDa
GenBank accession numberNM_001003817.1
Gene symbol Erbb2
Gene ID (NCBI) 13866
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Her2, or ErbB2, is a member of the EGF receptor family of receptor tyrosine kinases. It has no ligand-binding domain of its own. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signaling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase.