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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Hexokinase 1 Monoclonal antibody, PBS Only

Hexokinase 1 Monoclonal Antibody for WB, IP, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig

Applications

WB, IP, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B4F9

Cat no : 68419-1-PBS

Synonyms

Brain form hexokinase, hexokinase 1, Hexokinase type I, HK I, HK1, HK1 ta, HK1 tb, HK1 tc, HKI, HXK1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Hexokinase 1 Monoclonal antibody (68419-1-Ig)

    WB analysis using 68419-1-Ig (same clone as 68419-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68419-1-Ig (HK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68419-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using Hexokinase 1 Monoclonal antibody (68419-1-Ig)

    IP experiment of HeLa using 68419-1-Ig (same clone as 68419-1-PBS)

    IP result of anti-Hexokinase 1 (IP:68419-1-Ig, 4ug; Detection:68419-1-Ig 1:2000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 68419-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Hexokinase 1 Monoclonal antibody (68419-1-Ig)

    IHC staining of mouse brain using 68419-1-Ig (same clone as 68419-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68419-1-Ig (Hexokinase 1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68419-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68419-1-PBS targets Hexokinase 1 in WB, IP, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Hexokinase 1 fusion protein Ag8015 相同性解析による交差性が予測される生物種
Full Name hexokinase 1
Calculated molecular weight 102 kDa
Observed molecular weight100 kDa
GenBank accession numberBC008730
Gene symbol HK1
Gene ID (NCBI) 3098
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The hexokinases (HKs) catalyze the first obligatory step in glucose metabolism to generate glucose-6-phosphate. This not only furthers glucose entry by maintaining the concentration gradient for facilitated glucose influx but also provides the first intermediate for essentially all major pathways using glucose. There are four conventional HK isoforms, HK1/2/3/4, encoded by four different genes. Most adult tissues express only HK1. Muscle and adipose tissue use HK2 for glycolysis, liver, and pancreatic beta cells express HK4 (also called glucokinase) and do not express HK1 or HK2. (PMID: 31434645, PMID: 31848318)