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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Hexokinase 2 Monoclonal antibody, PBS Only (Detector)

Hexokinase 2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A11C3

Cat no : 66974-1-PBS

Synonyms

HK2, HK 2, Hexokinase-B, Hexokinase-2, hexokinase2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Hexokinase 2 Monoclonal antibody (66974-1-Ig)

    WB analysis using 66974-1-Ig (same clone as 66974-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66974-1-Ig (Hexokinase 2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66974-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using Hexokinase 2 Monoclonal antibody (66974-1-Ig)

    IHC staining of mouse skeletal muscle using 66974-1-Ig (same clone as 66974-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66974-1-Ig (Hexokinase 2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66974-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Hexokinase 2 Monoclonal antibody (66974-1-Ig)

    IF Staining of HeLa using 66974-1-Ig (same clone as 66974-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Hexokinase 2 antibody (66974-1-Ig, Clone: 2A11C3 ) at dilution of 1:750 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66974-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using Hexokinase 2 Monoclonal antibody (66974-1-Ig)

    FC experiment of HepG2 using 66974-1-Ig (same clone as 66974-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Hexokinase 2 Monoclonal antibody (66974-1-Ig, Clone:2A11C3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66974-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50448-3

    Cytometric bead array standard curve of MP50448-3

    Cytometric bead array standard curve of MP50448-3, HK2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66974-4-PBS. Detection antibody: 66974-1-PBS. Standard:Ag16895. Range: 0.781-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66974-1-PBS targets Hexokinase 2 as part of a matched antibody pair:

MP50448-3: 66974-4-PBS capture and 66974-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Hexokinase 2 fusion protein Ag16895 相同性解析による交差性が予測される生物種
Full Name hexokinase 2
Calculated molecular weight 102 kDa
Observed molecular weight 102 kDa
GenBank accession numberBC021116
Gene symbol Hexokinase 2
Gene ID (NCBI) 3099
RRIDAB_2882294
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Hexokinase 2 (HK2), a rate-limiting enzyme in the first step of glycolysis pathway, expresses at high level in cancer cells compared with normal cells. HK2 provides a new target for cancer therapy due to its pivotal role in tumor tumourigenic and metastatic process. HK1 is constitutively expressed in most mammalian adult tissues. HK2, however, although is abundantly expressed in embryonic tissues, is expressed at high levels only in limited number of adult tissues such as adipose, skeletal, and cardiac muscles. (PMID: 29305912, PMID: 28427443, PMID: 23911236). The antibody also recognizes Hexokinase 1.