Validation Data Gallery
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Tested Applications
| Positive WB detected in | Cobalt Chloride treated NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82989-4-RR targets Hif-1 alpha in WB, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Hif-1 alpha fusion protein Ag33620 相同性解析による交差性が予測される生物種 |
| Full Name | hypoxia inducible factor 1, alpha subunit |
| Calculated molecular weight | 93 kDa |
| Observed molecular weight | 120 kDa |
| GenBank accession number | NM_001313919 |
| Gene Symbol | Hif1a |
| Gene ID (NCBI) | 15251 |
| RRID | AB_3670737 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q61221 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Hif-1 alpha antibody 82989-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |