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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Hif-1 alpha Recombinant antibody

Hif-1 alpha Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230101E8

Cat no : 82989-4-RR

Synonyms

Hif1a, 230101E8, Hypoxia inducible factor 1 alpha, HIF1α, HIF1alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using mouse Hif1a Recombinant antibody (82989-4-RR)

    WB analysis of NIH/3T3 using 82989-4-RR

    Cobalt Chloride treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82989-4-RR (Hif1a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82989-4-RR

    Affinity and Kinetic Characterization of 82989-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82989-4-RR against Mouse Hif1a were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inCobalt Chloride treated NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 82989-4-RR is Hif-1 alpha Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Hif-1 alpha fusion protein Ag33620 相同性解析による交差性が予測される生物種
Full Name hypoxia inducible factor 1, alpha subunit
Calculated molecular weight93 kDa
Observed molecular weight120 kDa
GenBank accession numberNM_001313919
Gene symbol Hif1a
Gene ID (NCBI) 15251
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Hif-1 alpha antibody 82989-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols