ChromoTek Histone-Chromobody® plasmid (eGFP)
DNA plasmid encoding for anti-H2A-H2B VHH (anti-H2A-H2B Nanobody) fused to EGFP.
Specificity
Histone
Applications
IF, Live cell imaging
Vector type
Mammalian expression vector
Reporter
EGFP
Cat no : tcg
Synonyms
Validation Data Gallery
Product Information
DNA plasmid encoding for anti-H2A-H2B VHH (anti-H2A-H2B Nanobody) fused to EGFP.
| Description |
The ChromoTek Histone-Chromobody* is a probe to visualize chromatin dynamics in live cells.
• Easily transfect your cells with the Histone-Chromobody plasmid • Histone-Chromobody as marker of chromatin, nucleosomes, and histones • Histone-Chromobody does not intercalate in DNA • Histone-Chromobody is non-toxic for cells over extended periods of time (up to 3 days) *Commercialized under a license of INRAE, the French National Research Institute for Agriculture, Food and Environment |
| Applications | Non-invasive live cell imaging Monitors chromatin dynamics Histone and chromatin as biomarker |
| Specificity | Histone H2A-H2B heterodimer, tested in mammalian cells, flies, and yeast |
| Reporter | EGFP |
| Vector type | Mammalian expression vector |
| Promoter | Constitutive CMV IE |
| Codon usage | Mammalian |
| Selection | Kan/Neo |
| Sequence | With the Histone-Chromobody plasmid you receive the sequence information of the alpaca antibody to H2A-H2B heterodimer fused to EGFP and the full vector sequence. |
| Transfection | Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Fisher Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type. |
| Microscopy techniques | Wide-field epifluorescence microscopy; confocal microscopy |
| Storage Condition | Shipped at ambient temperature. Store at -20 °C |
Documentation
| SDS |
|---|
| SDS Histone-Chromobody® EGFP plasmid (PDF) |
| Datasheet |
|---|
| Datasheet Histone-Chromobody® EGFP plasmid (PDF) |
| Brochure |
|---|
| Chromotek nanobodies brochure (PDF) |
| EULA |
|---|
| tcg End User License Agreement (EULA) Histone Chromobody EGFP plasmid non-profit (US) (PDF) |
| tcg End User License Agreement (EULA) Histone Chromobody EGFP plasmid non-profit (PDF) |
Publications
| Application | Title |
|---|---|
Nat Commun Helicase LSH/Hells regulates kinetochore function, histone H3/Thr3 phosphorylation and centromere transcription during oocyte meiosis. |