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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Histone H1.0 Recombinant antibody (83993-1-RR)

    WB analysis using 83993-1-RR (same clone as 83993-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83993-1-RR (Histone H1.0 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83993-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Histone H1.0 Recombinant antibody (83993-1-RR)

    WB analysis using 83993-1-RR (same clone as 83993-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83993-1-RR (Histone H1.0 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83993-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00895-1

    Sandwich ELISA standard curve of MP00895-1

    Sandwich ELISA standard curve of MP00895-1, Human Histone H1.0 Recombinant Matched Antibody Pair - PBS only. 83993-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag9982. 83993-1-PBS was HRP conjugated as the detection antibody. Range: 0.937-60 ng/mL

  • Sample test of MP00895-1

    Sample test of MP00895-1

    Hela cell lysate was measured. The human Histone H1.0 concentration of detected samples was determined to be 385.31 ng/mL (based on a 2.0 mg/mL extract load).

  • Flow cytometry (FC) experiment of BxPC-3 cells using Histone H1.0 Recombinant antibody (83993-1-RR)

    FC experiment of BxPC-3 using 83993-1-RR (same clone as 83993-1-PBS)

    1x10^6 BxPC-3 cells were intracellularly stained with 0.25 ug Histone H1.0 Recombinant antibody (83993-1-RR, Clone:241147D2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83993-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00895-1

    Cytometric bead array standard curve of MP00895-1

    Cytometric bead array standard curve of MP00895-1, Histone H1.0 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83993-3-PBS. Detection antibody: 83993-1-PBS. Standard: Ag9982. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP00895-3

    Cytometric bead array standard curve of MP00895-3

    Cytometric bead array standard curve of MP00895-3, Histone H1.0 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83993-2-PBS. Detection antibody: 83993-1-PBS. Standard: Ag9982. Range: 0.781-100 ng/mL.

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Histone H1.0 Recombinant antibody, PBS Only (Detector)

Histone H1.0 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83993-1-PBS
Clone No.241147D2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

H1F0, H1FV, H1-0, H10, H1 histone family, member 0

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83993-1-PBS targets Histone H1.0 as part of a matched antibody pair:

MP00895-1: 83993-3-PBS capture and 83993-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00895-3: 83993-2-PBS capture and 83993-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Histone H1.0 fusion protein Ag9982 相同性解析による交差性が予測される生物種
Full Name H1 histone family, member 0
Calculated molecular weight 21 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC000145
Gene Symbol Histone H1.0
Gene ID (NCBI) 3005
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07305
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Linker histones are involved in forming higher order structure in chromatin and maintaining overall chromatin compaction. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.Histone H1.0 (H1F0,H1FV) is a linker histone that is widely expressed in many tissues and almost all vertebrates, unlike some other linker histones. The observed molecular weight of H1F0 is about 32 kDa.