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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-Histone H2B (Ser14) Recombinant antibody (83811-1-RR)

    WB analysis using 83811-1-RR (same clone as 83811-1-PBS)

    Non-treated NIH/3T3 cells and PMA treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83811-1-RR (Phospho-Histone H2B (Ser14)) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 83811-1-PBS in a different storage buffer formulation.

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Phospho-Histone H2B (Ser14) Recombinant antibody, PBS Only

Phospho-Histone H2B (Ser14) Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83811-1-PBS
Clone No.240570A8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

HIST2H2BE, Histone H2B, H2BC21, H2B/q, H2B.1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83811-1-PBS targets Phospho-Histone H2B (Ser14) in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 2, H2be
Calculated molecular weight 14 kDa
GenBank accession numberBC005827
Gene Symbol Histone H2B
Gene ID (NCBI) 8349
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ16778
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.