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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Histone H3 Recombinant antibody, PBS Only

Histone H3 Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241079B2

Cat no : 81984-2-PBS

Synonyms

HIST2H3A, H3-clustered histone 14, H3-clustered histone 13, H3C15, H3C14

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Histone H3 Recombinant antibody (81984-2-RR)

    WB analysis using 81984-2-RR (same clone as 81984-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81984-2-RR (Histone H3 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81984-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Histone H3 Recombinant antibody (81984-2-RR)

    IF Staining of HeLa using 81984-2-RR (same clone as 81984-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Histone H3 antibody (81984-2-RR, Clone: 241079B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81984-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using Histone H3 Recombinant antibody (81984-2-RR)

    FC experiment of HepG2 using 81984-2-RR (same clone as 81984-2-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Histone H3 Recombinant antibody (81984-2-RR, Clone:241079B2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 81984-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81984-2-RR

    Affinity and Kinetic Characterization of 81984-2-RR

    Biolayer interferometry (BLl) kinetic assays of 81984-2-RR against Human Histone H3 were performed. The affinity constant is 12.2 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 81984-2-PBS is Histone H3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Histone H3 fusion protein Ag10644 相同性解析による交差性が予測される生物種
Full Name histone cluster 2, H3a
Calculated molecular weight 136 aa, 15 kDa
Observed molecular weight15 kDa
GenBank accession numberBC015544
Gene symbol Histone H3
Gene ID (NCBI) 333932
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Histone-H3, histone cluster 2, H3a is the core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machinery which requires DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone-H3 is expressed during S phase; then expression strongly decreases as cell division slows down during the process of differentiation.