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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Histone H3 (C-terminal) Monoclonal antibody, PBS Only

Histone H3 (C-terminal) Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, chicken, zebrafish, wheat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G10C3

Cat no : 68503-1-PBS

Synonyms

HIST1H3A, 2G10C3, Histone-H3, HistoneH3, Histone H3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Histone H3 (C-terminal) Monoclonal antibody (68503-1-Ig)

    WB analysis using 68503-1-Ig (same clone as 68503-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68503-1-Ig (HIST1H3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68503-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using Histone H3 (C-terminal) Monoclonal antibody (68503-1-Ig)

    IHC staining of mouse testis using 68503-1-Ig (same clone as 68503-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68503-1-Ig (HIST1H3A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68503-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using Histone H3 (C-terminal) Monoclonal antibody (68503-1-Ig)

    IHC staining of mouse testis using 68503-1-Ig (same clone as 68503-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68503-1-Ig (HIST1H3A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68503-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68503-1-PBS targets Histone H3 (C-terminal) in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, chicken, zebrafish, wheat samples.

Tested Reactivity human, mouse, rat, chicken, zebrafish, wheat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H3a
Observed molecular weight15 kDa
GenBank accession numberBC066245
Gene symbol HIST1H3A
Gene ID (NCBI) 8350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes.