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  • Featured Product
  • KD/KO Validated

HuR/ELAVL1 Monoclonal antibody, PBS Only

HuR/ELAVL1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A11H2

Cat no : 66549-1-PBS

Synonyms

ELAV like protein 1, ELAV1, ELAVL1, Hu antigen R, Hua, HuR, MelG

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using HuR/ELAVL1 Monoclonal antibody (66549-1-Ig)

    WB analysis of HEK-293 using 66549-1-Ig (same clone as 66549-1-PBS)

    WB result of HuR/ELAVL1 antibody (66549-1-Ig; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HuR transfected HEK-293 cells. This data was developed using the same antibody clone with 66549-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using HuR/ELAVL1 Monoclonal antibody (66549-1-Ig)

    WB analysis using 66549-1-Ig (same clone as 66549-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66549-1-Ig (HuR/ELAVL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66549-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using HuR/ELAVL1 Monoclonal antibody (66549-1-Ig)

    WB analysis using 66549-1-Ig (same clone as 66549-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66549-1-Ig (HuR/ELAVL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66549-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HuR/ELAVL1 Monoclonal antibody (66549-1-Ig)

    IHC staining of human breast cancer using 66549-1-Ig (same clone as 66549-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66549-1-Ig (HuR antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66549-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using HuR/ELAVL1 Monoclonal antibody (66549-1-Ig)

    IHC staining of human ovary tumor using 66549-1-Ig (same clone as 66549-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66549-1-Ig (HuR antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66549-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HuR/ELAVL1 Monoclonal antibody (66549-1-Ig)

    IHC staining of human breast cancer using 66549-1-Ig (same clone as 66549-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66549-1-Ig (HuR antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66549-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using HuR/ELAVL1 Monoclonal antibody (66549-1-Ig)

    IHC staining of human ovary tumor using 66549-1-Ig (same clone as 66549-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66549-1-Ig (HuR antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66549-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HuR/ELAVL1 Monoclonal antibody (66549-1-Ig)

    IF Staining of HepG2 using 66549-1-Ig (same clone as 66549-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HuR/ELAVL1 antibody (66549-1-Ig, Clone: 1A11H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66549-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66549-1-PBS targets HuR/ELAVL1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HuR/ELAVL1 fusion protein Ag2249 相同性解析による交差性が予測される生物種
Full Name ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)
Calculated molecular weight 326 aa, 36 kDa
Observed molecular weight 34-37 kDa
GenBank accession numberBC003376
Gene symbol HuR/ELAVL1
Gene ID (NCBI) 1994
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ELAVL1, also named as HUR, belongs to the RRM elav family. It is involved in 3'-UTR ARE-mediated MYC stabilization. ELAVL1 binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, ELAVL1 binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.