Human ATF4 ELISA Kit
Sensitivity
77.8 pg/mL
Range
93.8-6000 pg/mL
Reactivity
Human
Cat no : KE00147
Synonyms
CREB 2, CREB2, DNA binding protein TAXREB67, TAXREB67, TXREB
Validation Data Gallery
Product Information
KE00147 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The ATF4 ELISA kit is to be used to detect and quantify protein levels of endogenous ATF4. The assay recognizes human ATF4. An antibody specific for ATF4 has been pre-coated onto the microwells. The ATF4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for ATF4 is added to detect the captured ATF4 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human ATF4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysates |
| Assay type | Sandwich |
| Sensitivity | 77.8 pg/mL |
| Range | 93.8-6000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 468 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysates | 88% | 70%-113% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 279.9 | 18.0 | 6.4 |
| 2 | 20 | 878.4 | 44.9 | 5.1 |
| 3 | 20 | 3,600.8 | 170.7 | 4.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 290.2 | 24.1 | 8.3 |
| 2 | 24 | 883.5 | 56.3 | 6.4 |
| 3 | 24 | 3,553.9 | 139.6 | 3.9 |
Background Information
ATF4 is a transcription factor, that accumulates predominantly in osteoblasts, where it regulates terminal osteoblast differentiation and bone formation[PMID: 19016586]. As a basic leucine-zipper (bZip) transcription factor, ATF4 can regulate amino acid metabolism, cellular redox state, and anti-stress responses. It also regulates age-related and diet-induced obesity and glucose homeostasis in mammals, and has conserved metabolic functions in flies[PMID: 19726872]. Due to its location at chromosome 22q13, a region linked to schizophrenia, ATF4 is considered as a positional candidate gene for schizophrenia[PMID: 18163433]. Otherwise, since ATF4 is induced by tumour microenvironmental factors, and regulates processes relevant to cancer progression, it might serve as a potential therapeutic target in cancer.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CREB 2, CREB2, DNA binding protein TAXREB67, TAXREB67, TXREB |