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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Human B Cell Basics Panel

Host

Mouse

Reactivity

Human

Applications

FC

Conjugate

Various

Cat no : PK30008

Validation Data Gallery

  • 1 x 10^6 Human PBMCs were stained with PK30008 Human B Cell Basics Panel. CD45+ cells are gated. Parent population: lymphocytes. Cells were not fixed.

    1 x 10^6 Human PBMCs were stained with PK30008 Human B Cell Basics Panel. CD45+ cells are gated. Parent population: lymphocytes. Cells were not fixed.

  • 1 x 10^6 Human PBMCs were stained with PK30008 Human B Cell Basics Panel. CD3-/CD19+ cells are gated. Parent population: CD45+ lymphocytes. Cells were not fixed.

    1 x 10^6 Human PBMCs were stained with PK30008 Human B Cell Basics Panel. CD3-/CD19+ cells are gated. Parent population: CD45+ lymphocytes. Cells were not fixed.

  • 1 x 10^6 Human PBMCs were stained with PK30008 Human B Cell Basics Panel. CD20 and CD21 expression on CD45+CD3-CD19+ lymphocytes are shown. Cells were not fixed.

    1 x 10^6 Human PBMCs were stained with PK30008 Human B Cell Basics Panel. CD20 and CD21 expression on CD45+CD3-CD19+ lymphocytes are shown. Cells were not fixed.

Product Information

B lymphocytes are immune cells that produce antibodies to neutralize pathogens and foreign substances.

This panel is designed for the investigation of B lymphocytes (CD19 positive) and for the initial separation of the B lymphocyte population into further subpopulations by developmental stage. The PE channel is left open. 


Panel Components

Fluorochrome (ex/em)Catalog NoTargetCloneIsotypeDescription
FITC PlusFITC-65109            CD45HI30Mouse IgG1kLymphocyte marker
(495 nm/524 nm)
CoraLite Plus 750CL750-65151  CD3UCHT1Mouse IgG1kT cell marker
(755 nm/780 nm)
CoraLite Plus 647CL647-65197            CD194G7Mouse IgG1kB cell marker
(654 nm/674 nm)
Cardinal RedCR-65085            CD202H7Mouse IgG2bkB cell marker
(592 nm/611 nm)
Atlantic BlueAB-65198            CD21BU32Mouse IgG1kExpression varies depending on B cell development stage
(404 nm/458 nm)

Storage

Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.

Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.