Human/Rat BDNF ELISA Kit
Cat no : KE00096
Synonyms
BDNF precursor form, brain derived neurotrophic factor, Brain-derived neurotrophic factor
Validation Data Gallery
Product Information
KE00096 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human/rat BDNF ELISA kit is to be used to detect and quantify protein levels of endogenous human/rat BDNF. The assay recognizes human/rat BDNF. An antibody specific for human/rat BDNF has been pre-coated onto the microwells. The human/rat BDNF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human/rat BDNF is added to detect the captured human/rat BDNF protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Rat BDNF ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.1 pg/mL |
| Range | 12.5 - 800 pg/mL |
| Reactivity | Human/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 627 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 92% | 74%-117% |
| Cell culture supernatants | 90% | 73%-113% |
| Rat plasma | 96% | 72%-122% |
| Rat serum | 95% | 74%-125% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 35.8 | 2.3 | 6.3 |
| 2 | 20 | 116.5 | 9.2 | 7.9 |
| 3 | 20 | 513.9 | 36.9 | 7.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 32.2 | 2.6 | 8.1 |
| 2 | 24 | 108.6 | 8.2 | 7.6 |
| 3 | 24 | 517.3 | 40.8 | 7.9 |
Background Information
The brain-derived neurotrophic factor (BDNF), as a member of the neurotrophin family of growth factors, is broadly expressed in the central nervous system. BDNF is involved in cerebral morphogenesis, synaptic plasticity and learning, mental health, degenerative central nervous system (CNS) diseases, and energy metabolism. BDNF has recently been identified as one of the four major pharmaceutical targets in neuropsychiatric diseases, including depression and bipolar disorder. In addition, BDNF is increasingly considered as a potential therapeutic agent for neurodevelopmental disorders such as Rett syndrome.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | BDNF precursor form, brain derived neurotrophic factor, Brain-derived neurotrophic factor |
Publications
| Species | Sample Type | Title |
|---|---|---|
Phytomedicine Xiangshao Decoction alleviates gastric mucosal injury through NRF2 signaling pathway and reduces neuroinflammation in gastric ulcer rats | ||
Cell Rep BDNF exerts an NRF2-dependent cytoprotective function via a receptor-independent pathway. | ||
Biomed Pharmacother Rosemary extract activates oligodendrogenesis genes in mouse brain and improves learning and memory ability | ||
J Ethnopharmacol Neuroprotective effects of Chaihu-Longgu-Muli Decoction in an insomnia-depression comorbidity model via Galectin-3-dependent JAK2/STAT3 inhibition. | ||
Geroscience Transcriptomics and biochemical evidence of trigonelline ameliorating learning and memory decline in the senescence-accelerated mouse prone 8 (SAMP8) model by suppressing proinflammatory cytokines and elevating neurotransmitter release | ||
Geroscience A novel synthetic 3,4,5-tri-feruloylquinic acid enhances learning and memory via neurotrophin signaling in an aging model senescence-accelerated prone 8 mice |