Human CD20 ELISA Kit
Sensitivity
0.33 ng/mL
Range
0.78-50 ng/mL
Reactivity
Human
Cat no : KE00082
Synonyms
B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7
Validation Data Gallery
Product Information
KE00082 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CD20 ELISA kit is to be used to detect and quantify protein levels of endogenous CD20. The assay recognizes human CD20. A polyclonal antibody specific for CD20 has been pre-coated onto the microwells. The CD20 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for CD20 is added to detect the captured CD20 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.
| Product name | Human CD20 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell culture supernatant, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.33 ng/mL |
| Range | 0.78-50 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 931 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatant | 98% | 83%-122% |
| Cell lysate | 101% | 75%-121% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | ? ug/ml | ? | ?% |
| 2 | 20 | ? ug/ml | ? | ?% |
| 3 | 20 | ? ug/ml | ? | ?% |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | ? ug/ml | ? | ?% |
| 2 | 20 | ? ug/ml | ? | ?% |
| 3 | 20 | ? ug/ml | ? | ?% |
Background Information
CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7 |