Human CD8 Memory T Cell Isolation Kit
Cat no : KMS307
Validation Data Gallery
Product Information
Memory T cells are antigen-exposed T lymphocytes which would mediate fast and robust response upon repeat encounter with antigen. Human CD8 Memory T Cell Isolation kit is designed to isolate untouched CD8+ memory T lymphocytes which contains a biotin labeled antibody cocktail and streptavidin conjugated magnetic beads. Through incubation of samples with biotin antibody cocktail followed by incubation of streptavidin beads, the magnetically labeled non CD8+ memory cells will be retained in the tube by magnetic separator. The untouched CD8+ T memory cells are collected in supernatant. Any downstream application can be performed on CD8+ memory cells after separation, such as Flow Cytometry and in vitro culture.
Components
KMS307-10:
·MS307A-10: 300 µL Human CD8 Isolation Biotin Antibody Cocktail (CD4, CD19, CD14, CD56, CD45RA, CD20, CD11b, CD16, CD36, CD235a, CD123, TCR γδ)
·MS307B-10: 300µL streptavidin magnetic beads
KMS307-100:
·MS307A-100: 3mL Human CD8 Isolation Biotin Antibody Cocktail (CD4, CD19, CD14, CD56, CD45RA, CD20, 11b, CD16, CD36, CD235a, CD123, TCR γδ)
·MS307B-100: 3mL streptavidin magnetic beads
Storage
2-8°C
Storage buffer
PBS, pH7.4, 0.2% BSA and 0.05% Sodium Azide
Reactivity
Human
Beads diameter
2.7 µm
Recommend usage
30µL biotin labeled antibody cocktail and 30µL streptavidin beads for 1x107 PBMCs
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Matthias (Verified Customer) (07-04-2024) | The isolation worked quite well, with an easy handling and quick processing. Cell yield was as expected with a puirty of 80-85% of the expected cell type.
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