Human CXCL5 ELISA Kit
Sensitivity
9.1 pg/mL
Range
31.25-2000 pg/mL
Reactivity
Human
Cat no : KE00271
Synonyms
C X C motif chemokine 5, CXCL5, ENA 78, ENA 78(1 78), ENA78, SCYB5, Small inducible cytokine B5
Validation Data Gallery
Product Information
KE00271 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CXCL5 ELISA kit is to be used to detect and quantify protein levels of endogenous human CXCL5. The assay recognizes human CXCL5. An antibody specific for human CXCL5 has been pre-coated onto the microwells. The human CXCL5 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human CXCL5 is added to detect the captured human CXCL5 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CXCL5 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 9.1 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6374 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 83% | 71%-95% |
| Human plasma | 98% | 79%-117% |
| Cell culture supernatants | 99% | 77%-123% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 973.2 | 49.1 | 5.0 |
| 2 | 20 | 233.3 | 22.0 | 9.4 |
| 3 | 20 | 58.1 | 5.4 | 9.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 996.5 | 69.1 | 6.9 |
| 2 | 24 | 238.2 | 21.3 | 8.9 |
| 3 | 24 | 56.5 | 5.1 | 9.1 |
Background Information
CXCL5 is a member of the ELR+ CXC chemokine family, whose members contain a highly conserved three amino acid motif (ELR+) that promotes angiogenesis and is highly associated with aberrant angiogenesis. CXCL5 is an important chemokine secreted by immune cells, such as monocytes and T lymphocytes. Recent studies indicated that CXCL5 is aberrantly expressed in >14 different types of cancer, including hepatocellular carcinoma, prostate cancer, pancreatic cancer, and gastric cancer. Antibody neutralization of CXCL5 in experimental models of human non-small-cell lung cancer decreased tumor angiogenesis and metastasis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | C X C motif chemokine 5, CXCL5, ENA 78, ENA 78(1 78), ENA78, SCYB5, Small inducible cytokine B5 |