Human Endostatin ELISA Kit

Sensitivity

4.3 pg/mL

Range

62.5-4000 pg/mL

Reactivity

Human

Cat no : KE00259

Synonyms

COL18A1, Collagen alpha 1(XVIII) chain, collagen, type XVIII, alpha 1, endostatin, FLJ27325, FLJ34914, KNO, KNO1



Product Information

KE00259 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Endostatin ELISA kit is to be used to detect and quantify protein levels of endogenous human Endostatin. The assay recognizes human Endostatin. An antibody specific for human Endostatin has been pre-coated onto the microwells. The human Endostatin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human Endostatin is added to detect the captured human Endostatin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human Endostatin ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants, Saliva
Assay type Sandwich
Sensitivity 4.3 pg/mL
Range 62.5-4000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 80781

Recovery

Sample TypeAverageRange
Human serum109%96%-128%
Cell culture supernatants90%78%-103%
Saliva89%76%-98%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120947.423.92.5
220481.216.33.4
320109.05.44.9

InterAssay

Samplenmean ( pg/mL)SDCV%
124934.514.21.5
224488.311.42.3
324117.66.95.9

Background Information

Endostatin, a 20-kDa C-terminal fragment derived from collagen alpha-1(XVIII) chain (COL18A1), is an endogenously produced inhibitor of angiogenesis. Many different proteases are able to cleave the NC1 domain of collagen alpha-1(XVIII) chain to produce endostatin including cathepsins L, B, and K, elastase. Endostatin inhibits proliferation and migration of endothelial cells, angiogenesis, and tumor growth. It may interfere with the pro-angiogenic action of growth factors such as basic fibroblast growth factor (bFGF/FGF-2) and vascular endothelial growth factor (VEGF).

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms COL18A1, Collagen alpha 1(XVIII) chain, collagen, type XVIII, alpha 1, endostatin, FLJ27325, FLJ34914, KNO, KNO1

Publications

SpeciesSample TypeTitle
humancell culture supernatants

J Nanobiotechnology

ALPL regulates pro-angiogenic capacity of mesenchymal stem cells through ATP-P2X7 axis controlled exosomes secretion

Authors - Jiayi Dong