製品名:

AuthentiKine™ Human IFN-gamma ELISA Kit

試験方法:

1 X 96 well plate

測定サンプル対象:

Serum, Plasma, Cell culture superntants

アッセイ方法:

Sandwich

感度:

0.4 pg/mL

測定範囲:

7.8-500 pg/mL

測定可能動物種:

Human

適用アプリケーション:

Sandwich ELISA

回収率:

Sample Type	Average	Range
Human plasma	96%	84%-107%
Cell culture superntants	95%	89%-107%

製品概要

KE00146 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IFN-gamma ELISA kit is to be used to detect and quantify protein levels of endogenous IFN-gamma. The assay recognizes human IFN-gamma. An antibody specific for IFN-gamma has been pre-coated onto the microwells. The IFN-gamma protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for IFN-gamma is added to detect the captured IFN-gamma protein. For signal development, horseradish peroxidase (HRP)-conjugated is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

保存方法:

All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:

IFG, IFI, IFN gamma, IFNG, IFN-gamma, Immune interferon, Interferon gamma, interferon, gamma