AuthentiKine™ Human IL-12 p40 ELISA Kit
Sensitivity
0.47 pg/mL
Range
31.25-2000 pg/mL
Reactivity
Human
Cat no : KE00240
Synonyms
CLMF, CLMF p40, CLMF2, IL 12 subunit p40, IL 12B, IL12B, Interleukin 12 subunit beta, NKSF, NKSF2
Validation Data Gallery
Product Information
KE00240 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-12 p40 ELISA kit is to be used to detect and quantify protein levels of endogenous IL-12 p40. The assay recognizes human IL-12 p40. An antibody specific for IL-12 p40 has been pre-coated onto the microwells. The IL-12 p40 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for IL-12 p40 is added to detect the captured IL-12 p40 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | AuthentiKine™ Human IL-12 p40 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Human serum plasma, Human urine, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.47 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3593 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human Plasma | 92% | 80%-121% |
| Human urine | 95% | 85%-110% |
| Cell culture supernatants | 104% | 92%-126% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 965.3 | 20.8 | 2.2 |
| 2 | 20 | 209.6 | 3.4 | 1.6 |
| 3 | 20 | 49.8 | 1.5 | 3.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 953.9 | 33.7 | 3.5 |
| 2 | 24 | 202.0 | 6.4 | 3.2 |
| 3 | 24 | 48.7 | 2.0 | 4.0 |
Background Information
Interleukin-12 (IL-12), is a cytokine that is secreted by activated phagocytes and dendritic cells and that induces interferon-γproduction by natural-killer and T lymphocytes. IL-12 is a 75 kDa heterodimer composed of a 35 kDa subunit (IL-12A p35) and a 40 kDa subunit (IL-12B p40) that is secreted by a wide variety of antigen presenting cells (APCs), including phagocytes, B cells and Langerhans cells. IL-12 p40 has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of IL-12 p40 was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of IL-12 p40 has been reported to be associated with the severity of atopic and non-atopic asthma in children.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CLMF, CLMF p40, CLMF2, IL 12 subunit p40, IL 12B, IL12B, Interleukin 12 subunit beta, NKSF, NKSF2 |