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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IgG Heavy chain Monoclonal antibody

Human IgG Heavy chain Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1C6B4

Cat no : 67760-1-Ig

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Human IgG Heavy chain Monoclonal antibody (67760-1-Ig)

    WB analysis of human plasma using 67760-1-Ig

    16 nL human plasma were subjected to SDS PAGE followed by western blot with 67760-1-Ig (Human IgG Heavy chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Human IgG Heavy chain Monoclonal antibody (67760-1-Ig)

    IHC staining of human tonsillitis using 67760-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67760-1-Ig (Human IgG Heavy chain antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Human IgG Heavy chain Monoclonal antibody (67760-1-Ig)

    IHC staining of human tonsillitis using 67760-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67760-1-Ig (Human IgG Heavy chain antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Human IgG Heavy chain Monoclonal antibody (67760-1-Ig)

    IHC staining of human tonsillitis using 67760-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67760-1-Ig (Human IgG Heavy chain antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using Human IgG Heavy chain Monoclonal antibody (67760-1-Ig)

    IF Staining of human tonsillitis using 67760-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Human IgG Heavy chain antibody (67760-1-Ig, Clone: 1C6B4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inhuman plasma
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67760-1-Ig targets Human IgG Heavy chain in WB, IHC, IF-P, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen nature protein 相同性解析による交差性が予測される生物種
Full Name Human IgG Heavy chain
Calculated molecular weight 155 kDa
Observed molecular weight~50 kDa
Gene symbol
Gene ID (NCBI)
RRIDAB_2918528
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

This antibody detects the heavy chain of human IgG.

Protocols

Product Specific Protocols
WB protocol for Human IgG Heavy chain antibody 67760-1-IgDownload protocol
IHC protocol for Human IgG Heavy chain antibody 67760-1-IgDownload protocol
IF protocol for Human IgG Heavy chain antibody 67760-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols