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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Human IgG4 Monoclonal antibody (66408-1-Ig)

    WB analysis of human plasma using 66408-1-Ig (same clone as 66408-1-PBS)

    human plasma (0.4 nL) were subjected to SDS PAGE followed by western blot with 66408-1-Ig (human IgG4 Antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66408-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human skeletal muscle tissue using Human IgG4 Monoclonal antibody (66408-1-Ig)

    WB analysis of human skeletal muscle using 66408-1-Ig (same clone as 66408-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66408-1-Ig (IGHG4 Antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66408-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using Human IgG4 Monoclonal antibody (66408-1-Ig)

    WB analysis of human heart using 66408-1-Ig (same clone as 66408-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 66408-1-Ig (IGHG4 Antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66408-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Human IgG4 Monoclonal antibody (66408-1-Ig)

    IHC staining of human tonsillitis using 66408-1-Ig (same clone as 66408-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66408-1-Ig (Human IgG4 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66408-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Human IgG4 Monoclonal antibody (66408-1-Ig)

    IHC staining of human tonsillitis using 66408-1-Ig (same clone as 66408-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66408-1-Ig (Human IgG4 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66408-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human appendicitis tissue using Human IgG4 Monoclonal antibody (66408-1-Ig)

    IF Staining of human appendicitis using 66408-1-Ig (same clone as 66408-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Human IgG4 antibody (66408-1-Ig, Clone: 1A1A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66408-1-PBS in a different storage buffer formulation.

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Human IgG4 Monoclonal antibody, PBS Only

Human IgG4 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66408-1-PBS
Clone No.1A1A2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Synonyms

Human IgG4, IgG4, IGHG4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1mg/ml
100 μg, 1mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66408-1-PBS targets Human IgG4 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name immunoglobulin heavy constant gamma 4 (G4m marker)
Calculated molecular weight 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberAL928742
Gene Symbol IGHG4
Gene ID (NCBI) 3503
RRIDAB_2881780
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

This antibody detects the heavy chain of human IgG4. The immunogen of this antibody is the specific domain "NKGLPSSIEK" of IGHG4.