Human MHC class I ELISA Kit
Cat no : KE00035
Synonyms
HLA A, HLA Class I, HLA class I (HLA-A), HLAA, HLA-A, HLA-B, HLA-C, MHC class I antigen A*3
Validation Data Gallery
Product Information
KE00035 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The MHC classⅠELISA Kit is to be used to detect and quantify protein levels of endogenous MHC classⅠmolecules. The assay recognizes human MHC classⅠmolecules. An MHC classⅠantibody has been pre-coated onto the microwells. The MHC class Ⅰproteins in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for MHC class I is added to detect the captured MHC classⅠproteins. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human MHC class I ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 19.6 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3105 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 107% | 95%-128% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 126.8 | 10.1 | 8.0 |
| 2 | 20 | 942.0 | 67.2 | 7.1 |
| 3 | 20 | 1,416.3 | 101.3 | 7.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 146.6 | 15.2 | 10.4 |
| 2 | 24 | 1,085.7 | 72.1 | 6.6 |
| 3 | 24 | 1901.1 | 100.8 | 5.3 |
Background Information
Major histocompatibility complex (MHC) antigens, also know in human as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). MHC classⅠmolecules (HLA-A, -B and -C) are polymorphic membrane glycoproteins composed of two polypeptide chains, a heavy alpha chain encoded by variant HLA-A, -B or -C gene and a smaller beta chain (β2-microglobulin). They are peptide-binding proteins that present processed antigens to T lymphocytes. This ELISA kit (KE00035) can be used in the measurement of HLA-A, -B and -C in biological samples.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | HLA A, HLA Class I, HLA class I (HLA-A), HLAA, HLA-A, HLA-B, HLA-C, MHC class I antigen A*3 |
Publications
| Species | Sample Type | Title |
|---|---|---|
J Tissue Eng Regen Med A bioartificial kidney device with polarized secretion of immune modulators. | ||
Sci Rep Allostimulatory capacity of conditionally immortalized proximal tubule cell lines for bioartificial kidney application. | ||
Virulence Platelet-derived major histocompatibility complex class I coating on Treponema pallidum attenuates natural killer cell lethality |