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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Human Naïve/Memory T Cell Panel

Host

Mouse

Reactivity

Human

Applications

FC

Conjugate

Various

Cat no : PK30011

Validation Data Gallery

  • 1x10^6 Human PBMCs were stained with PK30011 Human Memory/Naïve T Cell Panel. CD3+ cells are gated. Parent population: Lymphocytes. Cells were not fixed.

    1x10^6 Human PBMCs were stained with PK30011 Human Memory/Naïve T Cell Panel. CD3+ cells are gated. Parent population: Lymphocytes. Cells were not fixed.

  • 1x10^6 Human PBMCs were stained with PK30011 Human Memory/Naïve T Cell Panel. CD4 and CD8 expression on CD3+ lymphocytes are shown. Cells were not fixed.

    1x10^6 Human PBMCs were stained with PK30011 Human Memory/Naïve T Cell Panel. CD4 and CD8 expression on CD3+ lymphocytes are shown. Cells were not fixed.

  • 1x10^6 Human PBMCs were stained with PK30011 Human Memory/Naïve T Cell Panel. CD45RA and CD45RO expression on CD3+CD8+ (left) and CD3+/CD4+ (right) lymphocytes are shown. Cells were not fixed.

    1x10^6 Human PBMCs were stained with PK30011 Human Memory/Naïve T Cell Panel. CD45RA and CD45RO expression on CD3+CD8+ (left) and CD3+/CD4+ (right) lymphocytes are shown. Cells were not fixed.

Product Information

Naïve T cells have not previously encountered antigen while memory T cells have and can therefore more quickly respond to subsequent encounters of that antigen.

This panel is designed for the separation of T lymphocytes (CD3 positive) into Helper T Lymphocyte (CD4 positive), Cytotoxic T Lymphocyte (CD8 positive), Naïve T Lymphocytes (CD45RA expressing) and Memory T Lymphocytes (CD45RO expressing). The PE channel is left open. 


Panel Components

Fluorochrome (ex/em) Catalog No Target Clone Isotype Description
CoraLite Plus 405 CL405-65151 CD3 UCHT1 Mouse IgG1k T cell marker
(399 nm/422 nm)
CoraLite Plus 647 CL647-65134   CD4 OKT4 Mouse IgG2bk T helper cell marker
(654 nm/674 nm)
CoraLite Plus 750 CL750-65135 CD8a OKT8 Mouse IgG2ak Cytotoxic T cell marker
(755 nm/780 nm)
Cardinal Red CR-65226 CD45RA F8-11-13 Mouse IgG1k Naïve T cell Marker
(592 nm/611 nm)
FITC Plus FITC-65150 CD45RO UCHL1 Mouse IgG2ak Memory T cell Marker
(495 nm/524 nm)

Storage

Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.

Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.