Human TF ELISA Kit
Sensitivity
0.6 ng/mL
Range
1.56-100 ng/mL
Reactivity
Human
Cat no : KE00046
Synonyms
Beta 1 metal binding globulin, PRO1557, PRO2086, Serotransferrin, Siderophilin, TF, transferrin
Validation Data Gallery
Product Information
KE00046 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TF ELISA kit is to be used to detect and quantify protein levels of endogenous TF. The assay recognizes human TF. An antibody specific for TF has been pre-coated onto the microwells. The TF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for TF is added to detect the captured TF protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human TF ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Urine |
| Assay type | Sandwich |
| Sensitivity | 0.6 ng/mL |
| Range | 1.56-100 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7018 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Urine | 88% | 79%-97% |
| Human plasma | 103% | 81%-126% |
| Cell culture supernatants | 102% | 90%-120% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 111.4 | 5.6 | 5.0 |
| 2 | 20 | 18.4 | 0.8 | 4.2 |
| 3 | 20 | 4.5 | 0.2 | 5.3 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 101.3 | 8.2 | 8.1 |
| 2 | 24 | 17.9 | 1.3 | 7.2 |
| 3 | 24 | 4.3 | 0.3 | 7.5 |
Background Information
Serotransferrin (TF) is a 77 kDa secreted protein which is also named as transferrin, siderophilin, beta-1 metal-binding globulin and belongs to a family of homologous iron-binding glycoproteins that encompasses lactoferrin (found both intracellular and in secretions, including milk), melanotrasferrin (present on melanoma cells) and ovotransferrin (present in egg white). TF is a multi-function protein with a primary role in transporting iron in a safe, redox-inactive state from absorption to utilization or storage sites around the body. The association of Tf with the immune system derives from its ability to restrict serum free-iron levels, creating low-iron environments where the infection capacity of pathogenic microorganisms is limited. TF is expressed predominantly in the liver and secreted in plasma, but lower amounts can be synthesized in other tissues such as the brain and the testis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Beta 1 metal binding globulin, PRO1557, PRO2086, Serotransferrin, Siderophilin, TF, transferrin |