Human Total IL-18 ELISA Kit
Cat no : KE00193
Synonyms
Iboctadekin, IFN gamma inducing factor, IGIF, IL 1 gamma, IL 18, IL 1g, IL18, IL-18, IL1F4, Interleukin 1 gamma, Interleukin 18
Validation Data Gallery
Product Information
KE00193 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Total IL-18 ELISA kit is to be used to detect and quantify protein levels of endogenous Total IL-18. The assay recognizes human Total IL-18. An antibody specific for Total IL-18 has been pre-coated onto the microwells. The Total IL-18 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human Total IL-18 is added to detect the captured human Total IL-18 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Total IL-18 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 0.3 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3606 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 109% | 98%-127% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 475.7 | 22.1 | 4.7 |
| 2 | 20 | 112.5 | 3.6 | 3.2 |
| 3 | 20 | 27.3 | 1.4 | 5.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 506.3 | 49.1 | 9.7 |
| 2 | 24 | 114.3 | 9.6 | 8.4 |
| 3 | 24 | 28.0 | 1.5 | 5.5 |
Background Information
IL-18 is a member of IL-1 superfamily of cytokines. IL-18 is expressed by many human lymphoid and nonlymphoid cells, and has an important role in inflammatory processes. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL-18 synergistically with IL-12 contributes to Th1 differentiation and, therefore, is important in host defense mechanisms against intracellular bacteria, viruses, and fungi. The IL-18 cytokine increased expression of vascular cell adhesion molecule-1(VCAM1) and the adherence of melanoma cells. IL-18 plays a major role in atherosclerotic plaque destabilization leading to acute ischemic syndromes. IL-18 negatively regulate NAFLD/NASH progression, as well as multiple aspects of metabolic syndrome via modulation of the gut microbiota.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Iboctadekin, IFN gamma inducing factor, IGIF, IL 1 gamma, IL 18, IL 1g, IL18, IL-18, IL1F4, Interleukin 1 gamma, Interleukin 18 |
Publications
| Species | Sample Type | Title |
|---|---|---|
Int J Biol Sci Cisplatin Induces Pyroptosis via Activation of MEG3/NLRP3/caspase-1/GSDMD Pathway in Triple-Negative Breast Cancer. | ||
Food Chem Toxicol Elaidic acid induced hepatocyte pyroptosis via autophagy-CTSB-NLRP3 pathway | ||
PLoS One miR-328-3p targets TLR2 to ameliorate oxygen-glucose deprivation injury and neutrophil extracellular trap formation in HUVECs via inhibition of the NF-κB signaling pathway | ||
Biochem Biophys Res Commun TMAO promotes vascular endothelial cell pyroptosis via the LPEAT-mitophagy pathway | ||
Bioengineered Adiponectin inhibits the activation of lung fibroblasts and pulmonary fibrosis by regulating the nuclear factor kappa B (NF-κB) pathway. | ||
Cell Death Discov Microtubule associated protein 4 (MAP4) phosphorylation reduces cardiac microvascular density through NLRP3-related pyroptosis. |