Human VCAM-1/CD106 ELISA Kit
Cat no : KE00163
Synonyms
CD106, DKFZp779G2333, INCAM 100, vascular cell adhesion molecule 1, vascular cell adhesion protein 1, VCAM 1, VCAM1
Validation Data Gallery
Product Information
KE00163 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The VCAM-1 ELISA kit is to be used to detect and quantify protein levels of endogenous VCAM-1. The assay recognizes human VCAM-1. An antibody specific for VCAM-1 has been pre-coated onto the microwells. The VCAM-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for VCAM-1 is added to detect the captured VCAM-1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human VCAM-1/CD106 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 1.7 pg/mL |
| Range | 125-8000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7412 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 100% | 82%-113% |
| Cell culture supernatants | 85% | 75%-105% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 2,573.5 | 66.7 | 2.6 |
| 2 | 20 | 893.2 | 19.3 | 2.2 |
| 3 | 20 | 209.0 | 8.8 | 4.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 2,472.2 | 69.7 | 2.8 |
| 2 | 24 | 772.0 | 42.8 | 5.5 |
| 3 | 24 | 193.4 | 9.9 | 5.1 |
Background Information
Vascular cell adhesion molecule 1 (VCAM-1), also known as CD106, is a transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM-1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells. Soluble VCAM-1 (sVCAM-1) has been found in addition to membrane-bound VCAM-1. Increased sVCAM-1 has been shown to correlate with disease activity in various entities including rheumatoid arthritis, systemic lupus erythematosus, and lupus nephritis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD106, DKFZp779G2333, INCAM 100, vascular cell adhesion molecule 1, vascular cell adhesion protein 1, VCAM 1, VCAM1 |
Publications
| Species | Sample Type | Title |
|---|---|---|
Immun Inflamm Dis KDM2B regulates inflammation and oxidative stress of sepsis via targeting NF-κB and AP-1 pathways |