Huntingtin Polyclonal antibody
Huntingtin Polyclonal Antibody for IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IHC, IF-P, ELISA
Conjugate
Unconjugated
Cat no : 27206-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive IHC detected in | human cerebellum tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human brain tissue, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27206-1-AP targets Huntingtin in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Huntingtin fusion protein Ag25922 相同性解析による交差性が予測される生物種 |
| Full Name | huntingtin |
| Calculated molecular weight | 348 kDa |
| GenBank accession number | NM_002111 |
| Gene symbol | Huntingtin |
| Gene ID (NCBI) | 3064 |
| RRID | AB_2880799 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HTT(huntingtin), also named HD and IT15, belongs to the huntingtin family. HTT may play a role in microtubule-mediated transport or vesicle function. Defects in HTT are the cause of Huntington's disease (HD) which is an autosomal dominant neurodegenerative disorder characterized by involuntary movements (chorea), general motor impairment, psychiatric disorders, and dementia.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Huntingtin antibody 27206-1-AP | Download protocol |
| IF protocol for Huntingtin antibody 27206-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Florian (Verified Customer) (03-22-2024) | Primary antibody was tested for ICC, where no specific signal was observed. The antibody therefore was not able to specifically stain Huntingtin aggregates. We then also proceeded to detection of a WB, which resulted in a strong non-specific band. As the theoretical epitope of this antibody is of great interest for us, we would be very looking forward for improvements in this regard.
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