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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IAH1 Polyclonal antibody

IAH1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 17808-1-AP

Synonyms

IAH1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse testis tissue using IAH1 Polyclonal antibody (17808-1-AP)

    WB analysis of mouse testis using 17808-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 17808-1-AP (IAH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using IAH1 Polyclonal antibody (17808-1-AP)

    WB analysis of mouse brain using 17808-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17808-1-AP (IAH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using IAH1 Polyclonal antibody (17808-1-AP)

    WB analysis of mouse kidney using 17808-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17808-1-AP (IAH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using IAH1 Polyclonal antibody (17808-1-AP)

    IP experiment of mouse kidney using 17808-1-AP

    IP result of anti-IAH1 (IP:17808-1-AP, 3ug; Detection:17808-1-AP 1:500) with mouse kidney tissue lysate 5000ug.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using IAH1 Polyclonal antibody (17808-1-AP)

    IP experiment of mouse testis using 17808-1-AP

    IP result of anti-IAH1 (IP:17808-1-AP, 3ug; Detection:17808-1-AP 1:500) with mouse testis tissue lysate 4800ug.

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Tested Applications

Positive WB detected inmouse testis tissue, mouse brain tissue, mouse kidney tissue
Positive IP detected inmouse kidney tissue, mouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

17808-1-AP targets IAH1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IAH1 fusion protein Ag12089 相同性解析による交差性が予測される生物種
Full Name isoamyl acetate-hydrolyzing esterase 1 homolog (S. cerevisiae)
Calculated molecular weight 248 aa, 28 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC111025
Gene symbol IAH1
Gene ID (NCBI) 285148
RRIDAB_2123524
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IAH1 antibody 17808-1-APDownload protocol
IP protocol for IAH1 antibody 17808-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Proteomics

Increased expression of peroxiredoxin 1 and identification of a novel lipid-metabolizing enzyme in the early phase of liver ischemia reperfusion injury.

Authors - Wilson Claire H CH
View Article