Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 26942-1-AP
K-562 cells and U2OS cells were subjected to SDS PAGE followed by western blot with 26942-1-AP (IARS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 26942-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 26942-1-AP (IARS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of U2OS using 26942-1-AP
U2OS cells were subjected to SDS PAGE followed by western blot with 26942-1-AP (IARS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of U2OS using 26942-1-AP
IP result of anti-IARS (IP:26942-1-AP, 4ug; Detection:26942-1-AP 1:300) with U2OS cells lysate 1800 ug.
IHC staining of human testis using 26942-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26942-1-AP (IARS Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human testis using 26942-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26942-1-AP (IARS Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human liver cancer using 26942-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26942-1-AP (IARS Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver cancer using 26942-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26942-1-AP (IARS Antibody) at dilution of 1:200 (under 40x lens).

Featured Product
KD/KO Validated

IARS Polyclonal antibody

IARS Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 26942-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IF, IHC, ELISA

Synonyms

IARS, IARS1, IleRS, ILRS, IRS, Isoleucine tRNA ligase, isoleucyl tRNA synthetase, PRO0785

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (5)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	K-562 cells, U2OS cells
Positive IP detected in	U2OS cells
Positive IHC detected in	human testis tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IF	See 2 publications below

Product Information

26942-1-AP targets IARS in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, rat, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	IARS fusion protein Ag25686 相同性解析による交差性が予測される生物種
Full Name	isoleucyl-tRNA synthetase
Calculated molecular weight	144 kDa
Observed molecular weight	145 kDa
GenBank accession number	BC065552
Gene Symbol	IARS
Gene ID (NCBI)	3376
RRID	AB_2880693
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P41252
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for IARS antibody 26942-1-AP	Download protocol
IHC protocol for IARS antibody 26942-1-AP	Download protocol
IP protocol for IARS antibody 26942-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB	Food Chem Toxicol The crucial role of thioredoxin interacting protein in the liver insulin resistance induced by di (2-ethylhexyl) phthalates. Authors - Wang Zhang View Article
mouse	WB	Sci Rep Intermittent fasting activates macrophage migration inhibitory factor and alleviates high-fat diet-induced nonalcoholic fatty liver disease Authors - Dezhao Li View Article
human	WB,IF	Discov Oncol High dose isoleucine stabilizes nuclear PTEN to suppress the proliferation of lung cancer Authors - Haiqing Wang View Article
human	IF	Nat Commun Time-resolved proteomic profiling reveals compositional and functional transitions across the stress granule life cycle Authors - Shuyao Hu View Article
human	WB	Proc Natl Acad Sci U S A Multimodal cotranslational interactions direct assembly of the human multi-tRNA synthetase complex Authors - Krishnendu Khan KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 26942-1-AP

WB analysis of K-562 using 26942-1-AP

WB analysis of U2OS using 26942-1-AP

IP experiment of U2OS using 26942-1-AP

IHC staining of human testis using 26942-1-AP

IHC staining of human testis using 26942-1-AP

IHC staining of human liver cancer using 26942-1-AP

IHC staining of human liver cancer using 26942-1-AP

IARS Polyclonal antibody

IARS Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

The crucial role of thioredoxin interacting protein in the liver insulin resistance induced by di (2-ethylhexyl) phthalates.

Intermittent fasting activates macrophage migration inhibitory factor and alleviates high-fat diet-induced nonalcoholic fatty liver disease

High dose isoleucine stabilizes nuclear PTEN to suppress the proliferation of lung cancer

Time-resolved proteomic profiling reveals compositional and functional transitions across the stress granule life cycle

Multimodal cotranslational interactions direct assembly of the human multi-tRNA synthetase complex