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KD/KO Validated

IARS2 Polyclonal antibody

Name: IARS2 Polyclonal antibody
Brand: Proteintech
SKU: 17170-1-AP

IARS2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 17170-1-AP

Synonyms

IARS2, IleRS, Isoleucine tRNA ligase

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 17170-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17170-1-AP (IARS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 17170-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17170-1-AP (IARS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 17170-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 17170-1-AP (IARS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 17170-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 17170-1-AP (IARS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 17170-1-AP
IP result of anti-IARS2 (IP:17170-1-AP, 4ug; Detection:17170-1-AP 1:800) with HeLa cells lysate 4000ug.
IP experiment of Jurkat using 17170-1-AP
IP result of anti-IARS2 (IP:17170-1-AP, 4ug; Detection:17170-1-AP 1:800) with Jurkat cells lysate 4000ug.
IHC staining of human liver cancer using 17170-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17170-1-AP (IARS2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 17170-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17170-1-AP (IARS2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 17170-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17170-1-AP (IARS2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 17170-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17170-1-AP (IARS2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 17170-1-AP
Immunofluorescent analysis of HepG2 cells, using IARS2 antibody 17170-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Cat No. 17170-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, mouse lung tissue, mouse brain tissue, Jurkat cells
Positive IP detected in	HeLa cells, Jurkat cells
Positive IHC detected in	human liver cancer tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 5 publications below
IF	See 1 publications below

Product Information

17170-1-AP targets IARS2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	IARS2 fusion protein Ag10696 相同性解析による交差性が予測される生物種
Full Name	isoleucyl-tRNA synthetase 2, mitochondrial
Calculated molecular weight	1012 aa, 114 kDa
Observed molecular weight	114 kDa
GenBank accession number	BC010218
Gene symbol	IARS2
Gene ID (NCBI)	55699
RRID	AB_2264448
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family.

Protocols

Product Specific Protocols
WB protocol for IARS2 antibody 17170-1-AP	Download protocol
IHC protocol for IARS2 antibody 17170-1-AP	Download protocol
IF protocol for IARS2 antibody 17170-1-AP	Download protocol
IP protocol for IARS2 antibody 17170-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF, WB	Mol Cell Mitochondrial Threonyl-tRNA Synthetase TARS2 Is Required for Threonine-Sensitive mTORC1 Activation. Authors - Sung-Hoon Kim View Article
human	WB	Discov Oncol High dose isoleucine stabilizes nuclear PTEN to suppress the proliferation of lung cancer Authors - Haiqing Wang View Article
human	WB	Rev Assoc Med Bras (1992) IARS2 regulates proliferation, migration, and angiogenesis of human umbilical vein endothelial cells. Authors - Yue-Ming Yu View Article
human	WB	Nat Commun Targeted degradation of extracellular mitochondrial aspartyl-tRNA synthetase modulates immune responses Authors - Benjamin S Johnson View Article
human	WB	Front Oncol The Oncogene IARS2 Promotes Non-small Cell Lung Cancer Tumorigenesis by Activating the AKT/MTOR Pathway. Authors - Xin Di KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IARS2 Polyclonal antibody

IARS2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 17170-1-AP

WB analysis using 17170-1-AP

WB analysis of mouse lung using 17170-1-AP

WB analysis of mouse brain using 17170-1-AP

IP experiment of HeLa using 17170-1-AP

IP experiment of Jurkat using 17170-1-AP

IHC staining of human liver cancer using 17170-1-AP

IHC staining of human liver cancer using 17170-1-AP

IHC staining of human kidney using 17170-1-AP

IHC staining of human kidney using 17170-1-AP

IF Staining of HepG2 using 17170-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Mitochondrial Threonyl-tRNA Synthetase TARS2 Is Required for Threonine-Sensitive mTORC1 Activation.

High dose isoleucine stabilizes nuclear PTEN to suppress the proliferation of lung cancer

IARS2 regulates proliferation, migration, and angiogenesis of human umbilical vein endothelial cells.

Targeted degradation of extracellular mitochondrial aspartyl-tRNA synthetase modulates immune responses

The Oncogene IARS2 Promotes Non-small Cell Lung Cancer Tumorigenesis by Activating the AKT/MTOR Pathway.