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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IARS2 Recombinant antibody, PBS Only (Capture)

IARS2 Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

242219C5

Cat no : 84764-3-PBS

Synonyms

Isoleucyl-tRNA synthetase, Isoleucine--tRNA ligase, mitochondrial, Isoleucine tRNA ligase, IleRS, EC:6.1.1.5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IARS2 Recombinant antibody (84764-3-RR)

    WB analysis using 84764-3-RR (same clone as 84764-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84764-3-RR (IARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84764-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using IARS2 Recombinant antibody (84764-3-RR)

    WB analysis using 84764-3-RR (same clone as 84764-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84764-3-RR (IARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84764-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01543-3

    Sandwich ELISA standard curve of MP01543-3

    Sandwich ELISA standard curve of MP01543-3, Human IARS2 Recombinant Matched Antibody Pair - PBS only. 84764-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag10696. 84764-4-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

  • Sample test of MP01543-3

    Sample test of MP01543-3

    The mean IARS2 concentration was determined to be 92.84 ng/mL in HeLa cell extract based on a 1.00 mg/mL extract load.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using IARS2 Recombinant antibody (84764-3-RR)

    IF Staining of HepG2 using 84764-3-RR (same clone as 84764-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IARS2 antibody (84764-3-RR, Clone: 242219C5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84764-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01543-2

    Cytometric bead array standard curve of MP01543-2

    Cytometric bead array standard curve of MP01543-2, IARS2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84764-3-PBS. Detection antibody: 84764-1-PBS. Standard: Ag10696. Range: 0.313-20 ng/mL.

  • Cytometric bead array standard curve of MP01543-1

    Cytometric bead array standard curve of MP01543-1

    Cytometric bead array standard curve of MP01543-1, IARS2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84764-3-PBS. Detection antibody: 84764-2-PBS. Standard: Ag10696. Range: 0.313-20 ng/mL.

  • Affinity and Kinetic Characterization of 84764-3-RR

    Affinity and Kinetic Characterization of 84764-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84764-3-RR against Human IARS2 were performed. The affinity constant is 0.469 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84764-3-PBS targets IARS2 as part of a matched antibody pair:

MP01543-1: 84764-3-PBS capture and 84764-2-PBS detection (validated in Cytometric bead array)

MP01543-2: 84764-3-PBS capture and 84764-1-PBS detection (validated in Cytometric bead array)

MP01543-3: 84764-3-PBS capture and 84764-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IARS2 fusion protein Ag10696 相同性解析による交差性が予測される生物種
Full Name isoleucyl-tRNA synthetase 2, mitochondrial
Calculated molecular weight 1012 aa, 114 kDa
Observed molecular weight114 kDa
GenBank accession numberBC010218
Gene symbol IARS2
Gene ID (NCBI) 55699
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family.