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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IBA1 Polyclonal antibody

IBA1 Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 30523-1-AP

Synonyms

AI607846, AIF 1, Aif1, D17H6S50E, G1, Iba1

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of rat brain tissue using IBA1 Polyclonal antibody (30523-1-AP)

    IHC staining of rat brain using 30523-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30523-1-AP (IBA1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using IBA1 Polyclonal antibody (30523-1-AP)

    IHC staining of rat brain using 30523-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30523-1-AP (IBA1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using IBA1 Polyclonal antibody (30523-1-AP)

    IHC staining of mouse spleen using 30523-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30523-1-AP (IBA1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using IBA1 Polyclonal antibody (30523-1-AP)

    IHC staining of mouse spleen using 30523-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30523-1-AP (IBA1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using IBA1 Polyclonal antibody (30523-1-AP)

    IHC staining of mouse brain using 30523-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30523-1-AP (IBA1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using IBA1 Polyclonal antibody (30523-1-AP)

    IHC staining of mouse brain using 30523-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30523-1-AP (IBA1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using IBA1 Polyclonal antibody (30523-1-AP)

    IF Staining of rat brain using 30523-1-AP

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using IBA1 antibody (30523-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive IHC detected inrat brain tissue, mouse brain tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30523-1-AP targets IBA1 in IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IBA1 fusion protein Ag30575 相同性解析による交差性が予測される生物種
Full Name allograft inflammatory factor 1
Calculated molecular weight 17 kDa
GenBank accession numberNM_019467
Gene symbol IBA1
Gene ID (NCBI) 11629
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IBA1 is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Expression of IBA1 is up-regulated in activated microglia following facial nerve axotomy, ischemia, and several brain diseases, thereby implicating it in the activated phenotypes of microglia.

Protocols

Product Specific Protocols
IHC protocol for IBA1 antibody 30523-1-APDownload protocol
IF protocol for IBA1 antibody 30523-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols